Light-triggered green fluorescent protein silencing in human keratinocytes in culture using antisense oligonucleotides coupled to a photoreactive ruthenium(II) complex

Autor:	Nicolas Spinelli, Javiera Lambermont, Cécile Moucheron, Eric Defrancq, Andrée Kirsch-De Mesmaeker, Sébastien Bontems, Nancy Van Overstraeten-Schlögel, Martine Raes, Lionel Marcelis
Jazyk:	angličtina
Rok vydání:	2014
Předmět:	Oligonucleotide Chemistry Photochemistry Oligonucleotides chemistry.chemical_element General Chemistry In vitro Ruthenium Green fluorescent protein Electron transfer Biochemistry Gene silencing Cytotoxicity Antisense agents Polyacrylamide gel electrophoresis Conjugate
Zdroj:	ChemPlusChem. 79(11):1597-1604
ISSN:	2192-6506
DOI:	10.1002/cplu.201402212
Popis:	A photoreactive ruthenium(II) complex that contains two tetraazaphenanthrene (TAP) and one phenanthroline (phen) ligands was synthesized and then tethered to (antisense) oligonucleotides (Ru-ASO) to target a destabilized GFP (dGFP). The specificity of the photoreaction of this Ru-ASO conjugate was studied in vitro by polyacrylamide gel electrophoresis (PAGE) experiments in denaturing conditions. Other nonspecific Ru-ASO conjugates were also prepared and evaluated with human keratinocytes that expressed dGFP. An illumination-dependent cytotoxicity was observed for most Ru-ASO conjugates that varied from 10 to almost 40%, but only the specific Ru-ASO conjugate was able to significantly reduce GFP expression in illuminated cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c59831e06b6badc2f52481d3585123ce https://doi.org/10.1002/cplu.201402212 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.