Differential Distribution of cAMP Receptors cAR2 and cAR3 duringDictyosteliumDevelopment
| Autor: | Yimin Yu, Charles L. Saxe |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Time Factors
biology Gene Expression Regulation Developmental RNA Cell Biology biology.organism_classification Dictyostelium CAMP Receptors Molecular biology Receptors Cyclic AMP Cell biology Transformation Genetic CAMP signaling biology.protein Animals Distribution (pharmacology) Tissue Distribution Signal transduction Antibody Fluorescent Antibody Technique Indirect Receptor Molecular Biology Developmental Biology |
| Zdroj: | Developmental Biology. 173:353-356 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.1996.0030 |
| Popis: | Signal transduction via a family of cAMP receptor subtypes (cARs) is critical for proper development in the cellular slime moldDictyostelium.Genes encoding four related subtypes have been cloned and their expression, based on RNA accumulation, has been previously reported. Here we report the differential spatial and temporal distribution of cAR2 and cAR3 proteins, based on indirect double immunofluorescence. Cells were transformed with acarB::lacZconstruct, and an antibody against β-galactosidase was used to visualize cAR2 expression. Simultaneously, a cAR3-specific antibody was used to identify cAR3-expressing cells. Results indicate that by the time of tip formation (12–14 hr) both receptors are expressed and distribute in a virtually nonoverlapping pattern, with cAR2 being expressed on anterior, prestalk cells and cAR3 present in the rest of the organism. Differential distribution of these two receptor subtypes may result in distinct cAMP signaling mechanisms in the two major regions of the organism. |
| Databáze: | OpenAIRE |
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