Immunolabelling of the presynaptic membrane of Torpedo electric organ nerve terminals with an antiserum towards the acetylcholine releasing protein mediatophore
| Autor: | Guy Brochier, Maurice Israël, B. Lesbats |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Protein subunit
Schwann cell Nerve Tissue Proteins Biology Torpedo law.invention law Postsynaptic potential medicine Animals Active zone Microscopy Immunoelectron Calcimycin Nerve Endings Synaptosome Electric Organ Cholic Acids Cell Biology General Medicine Immunogold labelling Acetylcholine Cell biology Molecular Weight medicine.anatomical_structure Biochemistry Membrane protein Calcium Synaptosomes |
| Zdroj: | Biology of the Cell. 78:145-154 |
| ISSN: | 0248-4900 |
| DOI: | 10.1016/0248-4900(93)90125-x |
| Popis: | Mediatophore is a nerve terminal membrane protein purified from Torpedo electric organ on its ability to translocate acetylcholine upon calcium action. An antiserum able to immunoprecipitate mediatophore activity was used to study the subcellular distribution of this protein. The presynaptic membrane exhibited a strong and discontinuous immunogold labelling, especially at the active zone where ACh is thought to be released. Two antigens were recognized on immunoblots of synaptosomal membranes: the 15-kDa subunit of mediatophore and a 14-kDa membrane protein that has a wide non-neuronal distribution. Antibodies purified from the serum on native mediatophore and monospecific towards the 15-kDa antigen still gave a high presynaptic membrane localized labelling. In addition, a few 14-kDa protein sites were present at the active zone. The Schwann cell finger interposed between the presynaptic membrane and the postsynaptic arch also exhibited the 14-kDa antigen raising the question of a possible interaction of mediatophore with the 14-kDa protein originating from the Schwann cell. |
| Databáze: | OpenAIRE |
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