Differences in Tissue and Species Tropism of Reptarenavirus Species Studied by Vesicular Stomatitis Virus Pseudotypes

Autor:	Lev Levanov, Udo Hetzel, Juan Carlos de la Torre, Olli Vapalahti, Leonora Szirovicza, Teemu Smura, Yegor Korzyukov, Jussi Hepojoki, Anja Kipar, Luis Martinez-Sobrido, Rommel Paneth Iheozor-Ejiofor
Přispěvatelé:	Medicum, Viral Zoonosis Research Unit, Department of Virology, University of Helsinki, HUSLAB, Veterinary Pathology and Parasitology, Veterinary Biosciences, Faculty of Veterinary Medicine, Helsinki One Health (HOH), Veterinary Microbiology and Epidemiology, University Management, Olli Pekka Vapalahti / Principal Investigator, Helsinki University Hospital Area, University of Zurich, Hepojoki, Jussi
Rok vydání:	2020
Předmět:	0301 basic medicine viruses Cell lcsh:QR1-502 lcsh:Microbiology 0403 veterinary science Viral Envelope Proteins Chlorocebus aethiops LASSA FEVER Arenaviridae arenavirus 11832 Microbiology and virology STABLE SIGNAL PEPTIDE BIBD pseudotype tissue tropism Snakes 04 agricultural and veterinary sciences 3. Good health Infectious Diseases medicine.anatomical_structure VSV Vesicular stomatitis virus INCLUSION-BODY DISEASE S-RNA ALPHA-DYSTROGLYCAN reptarenavirus CELLULAR RECEPTOR 040301 veterinary sciences Green Fluorescent Proteins 10184 Institute of Veterinary Pathology ARENAVIRUS ENVELOPE GLYCOPROTEIN MEMBRANE-FUSION Biology Article Virus Cell Line 03 medical and health sciences Viral envelope Virology medicine Animals Humans Vero Cells Tropism Arenavirus Vesiculovirus 2725 Infectious Diseases biology.organism_classification LYMPHOCYTIC CHORIOMENINGITIS VIRUS Viral Tropism HEK293 Cells 030104 developmental biology A549 Cells Cell culture 2406 Virology JUNIN Tissue tropism 570 Life sciences biology 3111 Biomedicine
Zdroj:	Viruses Volume 12 Issue 4 Viruses, Vol 12, Iss 395, p 395 (2020)
ISSN:	1999-4915
DOI:	10.3390/v12040395
Popis:	Reptarenaviruses cause Boid Inclusion Body Disease (BIBD), and co-infections by several reptarenaviruses are common in affected snakes. Reptarenaviruses have only been found in captive snakes, and their reservoir hosts remain unknown. In affected animals, reptarenaviruses appear to replicate in most cell types, but their complete host range, as well as tissue and cell tropism are unknown. As with other enveloped viruses, the glycoproteins (GPs) present on the virion&rsquo s surface mediate reptarenavirus cell entry, and therefore, the GPs play a critical role in the virus cell and tissue tropism. Herein, we employed single cycle replication, GP deficient, recombinant vesicular stomatitis virus (VSV) expressing the enhanced green fluorescent protein (scrVSV∆G-eGFP) pseudotyped with different reptarenavirus GPs to study the virus cell tropism. We found that scrVSV∆G-eGFPs pseudotyped with reptarenavirus GPs readily entered mammalian cell lines, and some mammalian cell lines exhibited higher, compared to snake cell lines, susceptibility to reptarenavirus GP-mediated infection. Mammarenavirus GPs used as controls also mediated efficient entry into several snake cell lines. Our results confirm an important role of the virus surface GP in reptarenavirus cell tropism and that mamma-and reptarenaviruses exhibit high cross-species transmission potential.
Databáze:	OpenAIRE
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