Investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system
| Autor: | Daegyu Kim, Sun Min Kim, Tae-Joon Jeon, Ga-Lahm Park, Insu Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Skin Physiology Physiology Microfluidics lcsh:Medicine 02 engineering and technology Cell morphology Extracellular matrix Mice Animal Cells Cell Movement Lab-On-A-Chip Devices Medicine and Health Sciences Nanotechnology lcsh:Science Connective Tissue Cells Skin Multidisciplinary integumentary system Cell migration Equipment Design 021001 nanoscience & nanotechnology Cell Motility Connective Tissue Physical Sciences Engineering and Technology Fluidics Biological Cultures Cellular Types Anatomy Integumentary System 0210 nano-technology Research Article Materials science Surface Properties Materials Science Cell Migration Research and Analysis Methods 03 medical and health sciences Microfluidic channel Recovery function Tissue Repair Cell Adhesion Animals Process (anatomy) Materials by Attribute Nanomaterials Cell Proliferation Wound Healing lcsh:R Biology and Life Sciences Cell Biology Fibroblasts Cell Cultures Nanostructures 030104 developmental biology Biological Tissue NIH 3T3 Cells lcsh:Q Wound healing Physiological Processes Biomedical engineering Developmental Biology |
| Zdroj: | PLoS ONE, Vol 13, Iss 7, p e0201418 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | When living tissues are injured, they undergo a sequential process of homeostasis, inflammation, proliferation and maturation, which is called wound healing. The working mechanism of wound healing has not been wholly understood due to its complex environments with various mechanical and chemical factors. In this study, we propose a novel in vitro wound healing model using a microfluidic system that can manipulate the topography of the wound bed. The topography of the extracellular matrix (ECM) in the wound bed is one of the most important mechanical properties for rapid and effective wound healing. We focused our work on the topographical factor which is one of crucial mechanical cues in wound healing process by using various nano-patterns on the cell attachment surface. First, we analyzed the cell morphology and dynamic cellular behaviors of NIH-3T3 fibroblasts on the nano-patterned surface. Their morphology and dynamic behaviors were investigated for relevance with regard to the recovery function. Second, we developed a highly reproducible and inexpensive research platform for wound formation and the wound healing process by combining the nano-patterned surface and a microfluidic channel. The effect of topography on wound recovery performance was analyzed. This in vitro wound healing research platform will provide well-controlled topographic cue of wound bed and contribute to the study on the fundamental mechanism of wound healing. |
| Databáze: | OpenAIRE |
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