Lipoxygenase in Wheat: Genetic Control and Impact on Stability of Lutein and Lutein Esters
| Autor: | Shashi N. Goonetilleke, Judy Cheong, Daryl J. Mares, Diane E. Mather |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
endocrine system Lutein Health (social science) molecular markers Lutein esters Locus (genetics) Endogeny TP1-1185 Plant Science medicine.disease_cause 01 natural sciences Health Professions (miscellaneous) Microbiology Article Lipoxygenase chemistry.chemical_compound 0404 agricultural biotechnology medicine Food science Gene Mutation biology Chemical technology genetic loci food and beverages lipoxygenase genes 04 agricultural and veterinary sciences 040401 food science eye diseases Enzyme assay chemistry biology.protein sense organs Asian noodle colour 010606 plant biology & botany Food Science |
| Zdroj: | Foods Volume 10 Issue 5 Foods, Vol 10, Iss 1149, p 1149 (2021) |
| ISSN: | 2304-8158 |
| Popis: | Preservation of lutein concentrations in wheat-based end-products during processing is important both for product quality and nutritional value. A key constituent involved in lutein degradation is endogenous lipoxygenase. Lutein and lutein ester concentrations were compared at intervals during storage of noodle sheets prepared from flour of wheat varieties representing a range in lipoxygenase activity, as well as in different mill streams and in different grain tissues. Higher lipoxygenase concentration was associated with an increased loss of free lutein and lutein mono-esters whereas lutein diesters appeared to be more resistant to degradation. Lutein degradation was reduced in the presence of a lipoxygenase inhibitor, when noodle sheets were heated to destroy enzyme activity or when pH was increased. In addition, three populations were used to investigate the genetic control of lipoxygenase. A previously reported mutation of Lpx-B1.1 was associated with a reduction in activity from high to intermediate whilst a new locus on chromosome 4D was associated with variation between intermediate and near-zero. The gene underlying the 4D locus is a putative lipoxygenase. Stability of lutein could be improved by deployment of the mutations at the 4B and 4D loci and/or by post-harvest storage of grain under conditions that promote esterification. |
| Databáze: | OpenAIRE |
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