Non-flammable preparative reversed-phase liquid chromatography of recombinant human insulin-like growth factor-I
| Autor: | Robert L. Fahrner, Gregory S. Blank, Philip Lester, David Reifsnyder |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Flammable liquid
Chromatography Elution Organic Chemistry General Medicine Reversed-phase chromatography Biochemistry High-performance liquid chromatography Recombinant Proteins Analytical Chemistry Solvent chemistry.chemical_compound Column chromatography chemistry Humans Spectrophotometry Ultraviolet Thermoresponsive polymers in chromatography Insulin-Like Growth Factor I Acetonitrile Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 830:127-134 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(98)00866-8 |
| Popis: | Acetonitrile is used as an eluent for reversed-phase chromatography. However, because it is a flammable solvent, using acetonitrile on a large scale requires expensive equipment and facilities specially designed for flammable solvents. Using a non-flammable solvent as an eluent eliminates this expense. A method was developed to purify recombinant human insulin-like growth factor I by reversed-phase high-performance liquid chromatography using gradient elution with hexylene glycol, a non-flammable replacement for acetonitrile. The separation produced equivalent yield, purity and throughput as reversed-phase chromatography using elution with acetonitrile. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect