Association between memory B-cells and clinical and immunological features of primary Sjögren's syndrome and Sicca patients
| Autor: | Joana Cardigos, Catarina Martins, Nuno Alves, Luís Miguel Borrego, Glória Nunes, Carlos F. G. C. Geraldes, José Vaz-Patto, Ana Luísa Papoila, Filipe Barcelos, Jaime Branco, Teresa Lopes |
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| Rok vydání: | 2018 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Adult medicine.medical_specialty Disease duration Immunology medicine.disease_cause B-Lymphocytes / immunology Gastroenterology Autoimmunity B-Lymphocytes / cytology Pathogenesis Disease activity Diagnosis Differential 03 medical and health sciences 0302 clinical medicine stomatognathic system Rheumatology Internal medicine medicine Immunology and Allergy Humans 030203 arthritis & rheumatology B-Lymphocytes Receiver operating characteristic Sjogren's Syndrome / immunology business.industry HSAC OFT Sjogren's Syndrome / diagnosis medicine.disease Prognosis eye diseases stomatognathic diseases 030104 developmental biology Sjogren's Syndrome ROC Curve Case-Control Studies Female Sjogren s business Rheumatism |
| Zdroj: | Rheumatology international. 38(6) |
| ISSN: | 1437-160X |
| Popis: | B-cells play a pivotal role in primary Sjögren's syndrome (pSS) pathogenesis. We aim to (1) evaluate the distribution of B-lymphocyte subpopulations in pSS and Sicca patients, (2) establish cut-off points that discriminate pSS from controls, (3) evaluate the association between memory B-cells and phenotypic features in pSS. We included 57 pSS patients, 68 Sicca and 24 healthy controls. Circulating B-cells were characterized by flow cytometry as naïve and memory subsets and classified from Bm1 to Bm5. Compared to controls, pSS patients had lower percentages (29.5 vs 44.4%) and absolute numbers (47 vs 106 cells/µl) of memory B-cells. Through ROC curves, a cut-off of ≤ 58 total memory B-cells/µl yielded a specificity of 0.88 and a sensitivity of 0.60 for pSS, and was met by 59.6% of pSS patients, 38.8% of Sicca and 12.5% of controls. A cut-off of < 23.5 Switched-memory B-cells/µl yielded a specificity of 0.88 and a sensitivity of 0.54 and was met by 54.4% of pSS patients, 37.3% of Sicca and 12.5% of controls. In pSS, lower total memory B-cells count was associated with longer disease duration (14.3 vs 8.1 years, p = 0.006) and more active disease profile, as evaluated by the European League Against Rheumatism (EULAR) Sjögren's Syndrome Disease Activity Index (ESSDAI) (3.1 vs 1.4, p = 0.043). Decreased numbers of memory B-cells clearly discriminated pSS from controls and can also have prognostic value. It remains to be clarified whether Sicca patients with decreased memory B-cells represent pSS and if B-cell profiling could help in the diagnosis of pSS. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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