Transfer RNA Misidentification Scrambles Sense Codon Recoding
Autor: | Jiqiang Ling, Jesse Rinehart, John I. Glass, Laure Prat, Radha Krishnakumar, Hans R. Aerni, Dieter Söll, Chuck Merryman |
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Rok vydání: | 2013 |
Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Molecular Sequence Data Pyrrolysine Biology Biochemistry Article Amino Acyl-tRNA Synthetases Sense Codon chemistry.chemical_compound RNA Transfer Amino Acid Sequence Codon degeneracy Codon Molecular Biology chemistry.chemical_classification Genetics Mycoplasma capricolum Organic Chemistry beta-Galactosidase Genetic code Amino acid RNA Bacterial chemistry Codon usage bias Transfer RNA Molecular Medicine Genome Bacterial |
Zdroj: | ChemBioChem. 14:1967-1972 |
ISSN: | 1439-4227 |
Popis: | Sense codon recoding is the basis for genetic code expansion with more than two different noncanonical amino acids. It requires an unused (or rarely used) codon, and an orthogonal tRNA synthetase:tRNA pair with the complementary anticodon. The Mycoplasma capricolum genome contains just six CGG arginine codons, without a dedicated tRNA(Arg). We wanted to reassign this codon to pyrrolysine by providing M. capricolum with pyrrolysyl-tRNA synthetase, a synthetic tRNA with a CCG anticodon (tRNA(Pyl)(CCG)), and the genes for pyrrolysine biosynthesis. Here we show that tRNA(Pyl)(CCG) is efficiently recognized by the endogenous arginyl-tRNA synthetase, presumably at the anticodon. Mass spectrometry revealed that in the presence of tRNA(Pyl)(CCG), CGG codons are translated as arginine. This result is not unexpected as most tRNA synthetases use the anticodon as a recognition element. The data suggest that tRNA misidentification by endogenous aminoacyl-tRNA synthetases needs to be overcome for sense codon recoding. |
Databáze: | OpenAIRE |
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