An efficient Agrobacterium tumefaciens-mediated transformation method for Simplicillium subtropicum (Hypocreales: Cordycipitaceae)
| Autor: | Augusto Bartz Penteriche, Mariana Vieira Tomazett, Charley Christian Staats, Matheus da Silva Camargo, Clayton Luiz Borges, Augusto Schrank, Alexandre Melo Bailão, Nicolau Sbaraini, Célia Maria de Almeida Soares, Relber Aguiar Gonçales |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Genetics of Microorganisms Agrobacterium Hypocreales Simplicillium subtropicum Biology QH426-470 01 natural sciences 03 medical and health sciences Bioremediation Katushka Genetics Agrobacterium tumefaciens-mediated transformation Molecular Biology Gene 030304 developmental biology 0303 health sciences Agrobacterium tumefaciens biology.organism_classification Simplicillium Transformation (genetics) 010606 plant biology & botany Cordycipitaceae |
| Zdroj: | Genetics and Molecular Biology, Volume: 44, Issue: 3, Article number: e20210073, Published: 01 OCT 2021 Genetics and Molecular Biology, Vol 44, Iss 3 (2021) Genetics and Molecular Biology Genetics and Molecular Biology v.44 n.3 2021 Sociedade Brasileira de Genética (SBG) instacron:SBG |
| Popis: | Filamentous fungi are the organisms of choice for most industrial biotechnology. Some species can produce a variety of secondary metabolites and enzymes of commercial interest, and the production of valuable molecules has been enhanced through different molecular tools. Methods for genetic manipulation and transformation have been essential for the optimization of these organisms. The genus Simplicillium has attracted increased attention given several potential biotechnological applications. The Simplicillium genus harbors several entomopathogenic species and some isolates have been explored for bioremediation of heavy metal contaminants. Furthermore, the myriad of secondary metabolites isolated from Simplicillium spp. render these organisms as ideal targets for deep exploration and further biotechnological mining possibilities. However, the lack of molecular tools hampered the exploration of this genus. Thus, an Agrobacterium tumefaciens-mediated transformation method was established for Simplicillium subtropicum, employing the far-red fluorescent protein TURBOFP635/Katushka, as a visual marker, and the selection marker SUR gene, that confers resistance to chlorimuron ethyl. Notably, one round of transformation using the established method yielded almost 400 chlorimuron resistant isolates. Furthermore, these transformants displayed mitotic stability for, at least, five generations. We anticipate that this method can be useful for deep molecular exploration and improvement of strains in the Simplicillium genus. |
| Databáze: | OpenAIRE |
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