Biocide and antibiotic resistance of Enterococcus faecalis and Enterococcus faecium isolated from the swine meat chain
| Autor: | Sandra Torriani, Franca Rossi, Lucia Rizzotti |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Swine Enterococci Stress response genes Tetracycline medicine.drug_class Enterococcus faecium 030106 microbiology Antibiotics Biocide tolerance Sigma Factor Microbial Sensitivity Tests Microbiology Enterococcus faecalis 03 medical and health sciences Antibiotic resistance Bacterial Proteins Drug Resistance Multiple Bacterial Ampicillin medicine Animals Heat-Shock Proteins biology Chloramphenicol Membrane Transport Proteins Kanamycin biochemical phenomena metabolism and nutrition biology.organism_classification Anti-Bacterial Agents Erythromycin qac genes Red Meat 030104 developmental biology Genes Bacterial Disinfectants Food Science medicine.drug |
| Zdroj: | Food Microbiology. 60:160-164 |
| ISSN: | 0740-0020 |
| DOI: | 10.1016/j.fm.2016.07.009 |
| Popis: | In this study nine strains of Enterococcus faecalis and 12 strains of Enterococcus faecium, isolated from different sample types in the swine meat chain and previously characterized for the presence of antibiotic resistance genes, were examined for phenotypic tolerance to seven biocides (chlorexidine, benzalkonium chloride, triclosan, sodium hypochlorite, 2-propanol, formaldehyde and hydrogen peroxide) and resistance to nine antibiotics (ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol). Moreover, the presence of efflux system encoding genes qacA/B, qacC, qacE, qacEΔ1, emeA, and stress response genes, sigV and gsp65, involved in the tolerance to biocides, was analysed. Most strains were not tolerant to the biocides, but showed minimum inhibitory concentrations (MICs) higher than the recommended cut-off values for all the antibiotics tested, except for vancomycin and chloramphenicol. Only weak correlations, if any, were found between biocide and antibiotic resistance data. One E. faecalis strain was tolerant to triclosan and one E. faecium strain, with higher tolerance to chlorexidine than the other strains tested, was found to carry a qacA/B gene. Our results indicated that phenotypic resistance to antibiotics is very frequent in enterococcal isolates from the swine meat chain, but phenotypic tolerance to biocides is not common. On the other hand, the gene qacA/B was found for the first time in the species E. faecium, an indication of the necessity to adopt measures suitable to control the spread of biocide resistance determinants among enterococci. |
| Databáze: | OpenAIRE |
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