Changes in motor function and brain cortex mitochondrial active oxygen species production in aged mice
| Autor: | Analía G. Karadayian, Silvia Lores-Arnaiz, Paulina Lombardi, Juanita Bustamante, Rodolfo A. Cutrera |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Aging CIENCIAS MÉDICAS Y DE LA SALUD Cardiolipins AGEING CEREBRAL CORTEX Ciencias de la Salud SYNAPTOSOMES Mitochondrion Motor Activity Biochemistry 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Endocrinology Internal medicine Genetics medicine Cardiolipin NON-SYNAPTIC MITOCHONDRIA Animals Molecular Biology Cerebral Cortex MOTOR PERFORMANCE Chemistry Superoxide Brain cortex Cell Biology Acetylcholinesterase Mitochondria Active oxygen Otras Ciencias de la Salud 030104 developmental biology medicine.anatomical_structure Cerebral cortex Ageing Reactive Oxygen Species 030217 neurology & neurosurgery ACTIVE OXYGEN SPECIES Synaptosomes |
| Zdroj: | Experimental gerontology. 118 |
| ISSN: | 1873-6815 |
| Popis: | Neuronal ageing is a complex physiological process, associated to metabolic and motor changes. In this study, 3 and 17 months old male Swiss mice were used. Aged mice exhibited a significant reduction in motor performance and walking footprint pattern. Synaptosomes and mitochondrial fractions were isolated from mouse brain cortex. Active oxygen species and cardiolipin content were measured in both subcellular fractions. Synaptosomal acetylcholinesterase activity was measured in both animal age groups. Results showed that superoxide levels were 42.9% lower in synaptosomes from old mice as compared with young animals, while no changes were observed in non-synaptic mitochondria. Succinate-glutamate dependent H 2 O 2 production rate was 27.5% decreased in non-synaptic mitochondria from aged mice. Cardiolipin content was 21% decreased in synaptosomes from 17-months old animals, while no changes were observed in non-synaptic mitochondria. Acetylcholinesterase activity decreased 16% in 17-months old mice, as compared with young animals. Age-related alterations in neuronal function could be associated with changes in active oxygen species at synapses, with parallel motor deficiencies. Fil: Lores Arnaiz, Silvia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Lombardi, P.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Karadayian, Analia Graciela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Cutrera, Rodolfo Angel. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Bustamante, J.. Universidad Abierta Interamericana; Argentina |
| Databáze: | OpenAIRE |
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