Berberine reduces inflammation of human dental pulp fibroblast via miR-21/KBTBD7 axis
| Autor: | Sun Degang, Qi Cui, Song Jia, Qishan Wu, Fang Wang, Bingchang Xin, Jin Jiang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Lipopolysaccharide Berberine Cell Inflammation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Gene silencing Humans Fibroblast General Dentistry Dental Pulp Chemistry Cell growth Intracellular Signaling Peptides and Proteins NF-kappa B 030206 dentistry Cell Biology General Medicine Transfection Fibroblasts IκBα MicroRNAs 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology Cancer research Trans-Activators medicine.symptom Signal Transduction |
| Zdroj: | Archives of oral biology. 110 |
| ISSN: | 1879-1506 |
| Popis: | Objective This study is to investigate the effect and underlying mechanisms of berberine (BBR) on the proliferation and inflammatory levels of lipopolysaccharide induced human dental pulp fibroblast (LPS-HDPF). Methods Different concentrations of LPS were used to induce inflammatory response of HDPF. Cell proliferation was observed in BBR treated HDPF after transfection of miR-21 mimic, miR-21 inhibitor or sh-kBTBD7 plasmids and their negative controls. ELISA was employed to detect the expressions of inflammation related cytokines. Real-time quantitative RT-PCR was applied to estimate the expressions of miR-21 and KBTBD7. KBTBD7 expression was detected by Immunocytochemistry. Western blot analysis showed the protein levels of KBTBD7, Phospho-IKKα/β, IKKβ, Phospho-NF-κB p65, NF-κB p65, and IκBα. Luciferase reporter gene assay was used to determine the interaction between miR-21 and KBTBD7. Results LPS could promote inflammatory response in HDPF. Down-regulated miR-21 and up-regulated KBTBD7 were found in LPS-HDPF. BBR (25 uM) treatment in LPS-HDPF could ameliorate cell inflammatory response, presented by reduced expressions of IL-1β, IL-6 and TNF-α, as well as enhanced cell proliferation and miR-21 expression. Moreover, miR-21 negatively targets KBTBD7. Over-expression of miR-21 or silencing of KBTBD7 could enhance the protective role of BBR on LPS-HDPF by inhibiting inflammatory response and promoting cell proliferation. Transfection of miR-21 overexpression or KBTBD7 silencing in BBR treated LPS-HDPF could inhibit activation of NF-κB signal pathway. Conclusion Evidence in this study suggested that BBR mediates LPS induced inflammation in HDPF via miR-21/KBTBD7 axis to regulate NF-κB signal pathway, which may provide theoretical basis for BBR in prevention of pulpitis. |
| Databáze: | OpenAIRE |
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