Mass-Spectrometric Studies of the Interactions of Selected Metalloantibiotics and Drugs with Deprotonated Hexadeoxynucleotide GCATGC
| Autor: | Janna Anichina, Diethard K. Bohme |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Chemistry
Tandem mass spectrometry Mass spectrometric Intercalating Agents Surfaces Coatings and Films Bleomycin Crystallography Deprotonation Oligodeoxyribonucleotides Transition metal Metals Tandem Mass Spectrometry Duplex (building) Materials Chemistry Physical and Theoretical Chemistry Phenanthrolines |
| Zdroj: | The Journal of Physical Chemistry B. 113:328-335 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/jp807034v |
| Popis: | ESI tandem mass spectrometry is employed in a detailed study of the interactions of a hexameric duplex d(5'GCATGC) with three types of ligated first-row transition metal dications M(2+): metallated bleomycins, singly, doubly, and triply ligated metallophenanthrolines and [M(triethylenetetramine)](2+). The singly, doubly, and triply metallated species were found to dissociate by noncovalent separation into two strands with metal ions attached either to one or to both. Relative gas-phase stabilities of the double-stranded oligodeoxynucleotide (ODN)-M(2+) complexes were found to follow the order Mn(II)Fe(II)Co(II)Ni(II)Zn(II)Cu(II). Overall, the presence of metal dications is found to increase the gas-phase stability of the duplex against noncovalent dissociation with the exception of one and three copper dications. An analysis of the dissociation pathways and relative gas-phase stabilities of the species that were investigated provided a basis for the assessment of the possible binding modes between duplex oligonucleotides and metallocomplexes. |
| Databáze: | OpenAIRE |
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