Burn injury reduces neutrophil directional migration speed in microfluidic devices
| Autor: | Vijayakrishnan Ambravaneswaran, Daniel Irimia, M Bilodeau, Ronald G. Tompkins, Shawn P. Fagan, Mehmet Toner, Nitin Agrawal, Kathryn L. Butler |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Burn injury Neutrophils Fulminant Immunology/Innate Immunity lcsh:Medicine Motility Sepsis 03 medical and health sciences Young Adult 0302 clinical medicine Cell Movement Medicine Humans lcsh:Science Critical Care and Emergency Medicine/Trauma 030304 developmental biology Aged 0303 health sciences Multidisciplinary Innate immune system Thermal injury business.industry lcsh:R 030208 emergency & critical care medicine Chemotaxis Microfluidic Analytical Techniques Middle Aged medicine.disease Immunology lcsh:Q Female Biotechnology/Bioengineering business Burns Chemotaxis assay Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 7, p e11921 (2010) |
| ISSN: | 1932-6203 |
| Popis: | Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies had documented concurrent impairment in neutrophil chemotaxis with these post-burn inflammatory changes. While a few studies suggest that a link between neutrophil motility and patient mortality might exist, so far, cumbersome assays have prohibited exploration of the prognostic and diagnostic significance of chemotaxis after burn injury. To address this need, we developed a microfluidic device that is simple to operate and allows for precise and robust measurements of chemotaxis speed and persistence characteristics at single-cell resolution. Using this assay, we established a reference set of migration speed values for neutrophils from healthy subjects. Comparisons with samples from burn patients revealed impaired directional migration speed starting as early as 24 hours after burn injury, reaching a minimum at 72-120 hours, correlated to the size of the burn injury and potentially serving as an early indicator for concurrent infections. Further characterization of neutrophil chemotaxis using this new assay may have important diagnostic implications not only for burn patients but also for patients afflicted by other diseases that compromise neutrophil functions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|