Box-Behnken design optimization of mucoadhesive chitosan-coated nanoemulsions for rosmarinic acid nasal delivery—In vitro studies
Autor: | Helder Ferreira Teixeira, Morgana Dal Prá, Roselena Silvestri Schuh, Valquiria Linck Bassani, Elizandra Braganhol, Amélia T. Henriques, Letícia Scherer Koester, Flávia Nathiely Silveira Fachel, Bruna Medeiros-Neves, Kleyton Santos Veras |
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Rok vydání: | 2018 |
Předmět: |
Chromatography
food.ingredient Polymers and Plastics Rosmarinic acid Organic Chemistry Mucous membrane of nose 02 engineering and technology Penetration (firestop) Permeation 021001 nanoscience & nanotechnology 030226 pharmacology & pharmacy Box–Behnken design Lecithin Chitosan 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food chemistry Materials Chemistry Mucoadhesion 0210 nano-technology |
Zdroj: | Carbohydrate Polymers. 199:572-582 |
ISSN: | 0144-8617 |
Popis: | Mucoadhesive chitosan-coated nanoemulsions for rosmarinic acid (RA) nasal delivery were optimized. The optimum ratio between the formulation components that led to minimum droplet size and PDI, and maximal ζ-potential and RA content was obtained using Box-Behnken design (BBD). Optimized conditions were 8.5% oil phase (w/v), 3:10 lecithin to oil phase ratio (w/w), and 0.1% chitosan final concentration (w/v). Physicochemical characterization, mucoadhesion measurement, in vitro release and permeation/retention were performed. Optimized chitosan-coated RA nanoemulsions presented adequate physicochemical characteristics, high mucoadhesive potential, prolonged drug release, and long-lasting permeation time with a higher RA penetration/retention through porcine nasal mucosa. Cell viability and death by necrosis in fibroblasts cells were also evaluated to investigate the formulations safety. Formulations did not induce cytotoxicity following 24 h (3.125–50 μM) or 48 h (3.125–25 μM) of treatments. Overall results demonstrated that optimized chitosan-coated nanoemulsion showed to be a suitable carrier for RA nasal delivery aiming neuroprotective therapies. |
Databáze: | OpenAIRE |
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