Magnetic Resonance Imaging and Morphometric Quantitation of Cartilage Histology after Chronic Infusion of Interleukin 1 in Rabbit Knees
| Autor: | E. D. Roberts, J. Gronlund-Jacob, Elizabeth O'Byrne, G. DiPasquale, V. Blancuzzi, P. K. Paul, D. Wilson, K. Vosbeck |
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| Rok vydání: | 1993 |
| Předmět: |
Cartilage
Articular Male Pathology medicine.medical_specialty Arthritis General Biochemistry Genetics and Molecular Biology Bone resorption medicine Animals Humans Infusions Parenteral Lagomorpha medicine.diagnostic_test biology business.industry Cartilage Synovial Membrane Interleukin Magnetic resonance imaging Histology biology.organism_classification medicine.disease Magnetic Resonance Imaging Recombinant Proteins Staining medicine.anatomical_structure Rabbits business Interleukin-1 |
| Zdroj: | Experimental Biology and Medicine. 203:30-37 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-203-43569 |
| Popis: | Cartilage pathology in rabbit knees was monitored by noninvasive magnetic resonance imaging (MRI) and evaluated using morphometric histologic measurements. Infusion of rabbit knees with the cytokine interleukin 1 induces cartilage degradation and inflammation. A miniosmotic pump was implanted subcutaneously to deliver interleukin 1 through a polyethylene catheter inserted into the rabbit knee. Rabbit knees were imaged using MRI and prepared for histologic examination at 5 and 12 days after chronic infusion of interleukin 1. MRI obtained 0.7-mm sections for three-dimensional reconstruction of cartilage image. Cartilage deterioration near the site of infusion was visible on MRI. MRI measurements indicated a reduction in cartilage thickness. Histology revealed a loss of staining of cartilage matrix proteoglycan, synovial hypertrophy, and perichondral bone resorption. Morphometric analysis of cartilage histology indicated a reduction in both cellularity (chondrocytes/m mu 2 area) and cell to matrix area ratio. These observations suggest that a loss of proteoglycan, an early event in cartilage degeneration, can be detected by MRI. |
| Databáze: | OpenAIRE |
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