Structural basis of cycloaddition in biosynthesis of iboga and aspidosperma alkaloids
| Autor: | Sarah E. O'Connor, Ivo Jose Curcino Vieira, Clare E. M. Stevenson, Kate Bussey, Lorenzo Caputi, Jakob Franke, David M. Lawson, Scott C. Farrow |
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| Rok vydání: | 2019 |
| Předmět: |
Aspidosperma
Stereochemistry Tabernaemontana Carbazoles Single step Article Indole Alkaloids 03 medical and health sciences chemistry.chemical_compound Alkaloids Biosynthesis Hydrolase Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Cycloaddition Reaction 030302 biochemistry & molecular biology Substrate (chemistry) Cell Biology Plants biology.organism_classification Cycloaddition Mutational analysis Enzyme chemistry |
| Zdroj: | Nature chemical biology Nature Chemical Biology |
| ISSN: | 1552-4469 |
| Popis: | Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity. |
| Databáze: | OpenAIRE |
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