Single layer graphene functionalized MEA for enhanced detection of neuronal network development
| Autor: | Sandeep Keshavan, Neeraj Mishra, Michela Chiappalone, Silvia Dante, Camilla Coletti, Amira El Merhie, Daisuke Ito, Ilaria Colombi, Alberto Diaspro, Vaidotas Miseikis |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Synaptogenesis 02 engineering and technology law.invention 03 medical and health sciences Bursting 0302 clinical medicine law Materials Chemistry Biological neural network medicine Patch clamp Electrical and Electronic Engineering Instrumentation Graphene Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Coupling (electronics) Electrophysiology medicine.anatomical_structure Biophysics Neuron 0210 nano-technology 030217 neurology & neurosurgery |
| Zdroj: | Sensors and Actuators B: Chemical. 277:224-233 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2018.08.142 |
| Popis: | The exploitation of graphene for neuro-interfacing applications requires a complete, yet missing, understanding of neuron-graphene interaction. Here, we have explored the interplay between the carbon based interface and neuronal networks during the complete developmental phase at whole network scale. To this purpose, we have, first, successfully transferred large grains single layer graphene (LG-SLG) via wet etching onto commercial planar 60 electrode devices; then, we have compared to control the neuronal growth on the functionalized devices, recording the spontaneous activity up to completion of network maturation, i.e., from 7 to 25 days-in-vitro. The immunohistochemistry investigation demonstrated a comparable morphology of the neuronal network on SLG and control substrates but with a higher number of neurons on SLG. The most striking results of the electrophysiological investigation were the observation of spikes and bursts activity at an earlier developmental phase and of strongly synchronized neuronal networks on SLG-MEA versus control, suggesting an improved neuron/electrode coupling. These observations agree with our previous study of single neuron synaptogenesis by patch clamp, where earlier synaptogenesis on SLG compared to the control was detected. The results have been corroborated by the firing and bursting analysis showing higher and statistically significant values on SLG-MEA. |
| Databáze: | OpenAIRE |
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