Newcastle disease virus-induced cytopathic effect in infected cells is caused by apoptosis
| Autor: | S. K. Palia, Uttara Chaturvedi, P.V. Ravindra, Ashok K. Tiwari, R.S. Chauhan, Barkha Ratta |
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| Rok vydání: | 2009 |
| Předmět: |
Cancer Research
Programmed cell death Newcastle Disease viruses Newcastle disease virus Gene Expression Apoptosis DNA Fragmentation Biology Newcastle disease Cytopathogenic Effect Viral Virology Chlorocebus aethiops Animals Vero Cells Cytopathic effect Cell fusion biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology Infectious Diseases Proto-Oncogene Proteins c-bcl-2 Cell culture Caspases Vero cell DNA fragmentation |
| Zdroj: | Virus Research. 141:13-20 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2008.12.008 |
| Popis: | The velogenic Newcastle disease virus (NDV) causes highly infectious and economically significant Newcastle disease (ND) in birds of various species. In cell culture NDV induces cytopathic effect (CPE) characterized by rounding, vacuolation, syncytia formation and cell death. Aside from cell to cell fusion caused by the F and HN glycoprotein of the virus molecular events leading to cell death are not known. In the current study, NDV-infected Vero cells, at 48 h p.i., showed nuclear condensation, cytoplasm blebbing, DNA fragmentation, and phosphatidylserine translocation to the cell surface. In addition, virus-infected cells demonstrated decreased DNA content and an increased Bax to Bcl-2 ratio, p53 level and caspase 3, 8, 9 expression compared to mock-infected cells. Based on these results, it was concluded that CPE in NDV-infected cells was caused by to the induction of apoptosis with the involvement of p53 and the Bax, dependent apoptotic pathways. |
| Databáze: | OpenAIRE |
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