Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogen Candida albicans
| Autor: | C E Birse, Paul S. Sypherd, W A Fonzi, M Y Irwin |
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| Rok vydání: | 1993 |
| Předmět: |
Genes
Fungal Molecular Sequence Data Immunology Gene Expression Biology Microbiology Fungal Proteins Candida albicans Gene expression Genomic library Amino Acid Sequence Cloning Molecular Gene Gene Library Base Sequence cDNA library biology.organism_classification Molecular biology Corpus albicans Yeast Phenotype Infectious Diseases Parasitology Candidalysin Research Article |
| Zdroj: | Infection and Immunity. 61:3648-3655 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.61.9.3648-3655.1993 |
| Popis: | The gene ECE1 (extent of cell elongation 1) was isolated by differential hybridization screening of a Candida albicans cDNA library by using probes derived from populations of yeast cells or hyphae. Expression of this gene was not detected when C. albicans grew as a budding yeast cell but was observed within 30 min after cells had been induced to form hyphae. In all strains tested, regardless of the induction signal, ECE1 expression correlated with the extent of cell elongation. The genomic version of ECE1 was cloned and sequenced. The deduced 271-amino-acid polypeptide consisted of eight tandem repeats of a degenerate 34-amino-acid sequence which contained no discernible homology with other known sequences. An ECE1 null mutant displayed no morphological alterations, which demonstrated that ECE1 is not essential for cell elongation or hypha formation despite the strict morphological association of its expression. |
| Databáze: | OpenAIRE |
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