Increased Expression of CD16, CD69, and Very Late Antigen-1 on Blood Monocytes in Active Sarcoidosis
Autor: | Jules M.M. van den Bosch, Marcel Veltkamp, Anke M.E. Claessen, Heleen van Velzen-Blad, M. Heron, Jan C. Grutters |
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Rok vydání: | 2008 |
Předmět: |
Adult
Antigens Differentiation T-Lymphocyte Male Pulmonary and Respiratory Medicine Systemic disease Pathology medicine.medical_specialty Peptidyl-Dipeptidase A CD16 Lymphocyte Activation Critical Care and Intensive Care Medicine Severity of Illness Index Monocytes Immunophenotyping Integrin alpha1beta1 Proinflammatory cytokine Young Adult Immune system Sarcoidosis Pulmonary Antigen Antigens CD Humans Medicine Lectins C-Type Aged Immunity Cellular business.industry Monocyte Receptors IgG Middle Aged Flow Cytometry medicine.disease medicine.anatomical_structure Immunology Female Sarcoidosis Cardiology and Cardiovascular Medicine business Biomarkers Follow-Up Studies |
Zdroj: | Chest. 134:1001-1008 |
ISSN: | 0012-3692 |
Popis: | Different types of immune cells are involved in the formation of granulomas, a hallmark of pulmonary sarcoidosis. Proinflammatory monocytes are activated circulating monocytes thought to be related to the initial events of granuloma formation. We tested the hypothesis that peripheral blood monocytes in patients with active pulmonary sarcoidosis have an activated phenotype and, secondly, that measuring this activation status can provide a new tool for monitoring disease activity.Blood was collected of 23 steroid-naive patients presenting with pulmonary sarcoidosis and 10 healthy control subjects. Expression of CD16 (Fc-gamma type III receptor), CD69 (a general activation marker of cells of the hematopoietic lineage), and the integrin very late antigen (VLA)-1 (on interaction with extracellular matrix compounds mediates cell adhesion) was measured by flow cytometry.Percentages of monocytes expressing CD16, CD69, and VLA-1 in patients vs control subjects were 56.2 +/- 4.1% vs 12.2 +/- 2.4% (p0.0001), 87.3 +/- 2.1% vs 8.6 +/- 3.3% (p0.0001), and 66.5 +/- 3.6% vs 11.2 +/- 2.3% (p0.0001), respectively. Moreover, the CD69+VLA-1+ monocyte subset, abundantly present at disease presentation, was found to decrease to normal levels during follow-up with disease remission.Peripheral blood monocytes from patients with pulmonary sarcoidosis show a highly activated phenotype. Phenotyping circulating monocytes might be a promising tool for monitoring sarcoidosis disease activity but needs further investigation. |
Databáze: | OpenAIRE |
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