Identifying specific light inputs for each subgroup of brain clock neurons in Drosophila larvae
| Autor: | André Klarsfeld, François Rouyer, Elisabeth Chélot, Carine Vias, Marie Picot |
|---|---|
| Přispěvatelé: | Neurobiologie et Développement (N&eD), Centre National de la Recherche Scientifique (CNRS), Institut de Neurobiologie Alfred Fessard (INAF) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Rhodopsin
Opsin endocrine system Photoperiod media_common.quotation_subject Mutant Circadian clock CLOCK Proteins Neuropeptide Biology Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Cryptochrome Animals Drosophila Proteins Contrast (vision) Photopigment 030304 developmental biology media_common Neurons 0303 health sciences Behavior Animal General Neuroscience fungi Brain Articles Anatomy Cell biology Clock network nervous system Larva Drosophila Photoreceptor Cells Invertebrate [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Photic Stimulation 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroscience Journal of Neuroscience, Society for Neuroscience, 2011, 31 (48), pp.17406-15. ⟨10.1523/JNEUROSCI.5159-10.2011⟩ |
| ISSN: | 0270-6474 1529-2401 |
| Popis: | InDrosophila, opsin visual photopigments as well as blue-light-sensitive cryptochrome (CRY) contribute to the synchronization of circadian clocks. We focused on the relatively simple larval brain, with nine clock neurons per hemisphere: five lateral neurons (LNs), four of which express the pigment-dispersing factor (PDF) neuropeptide, and two pairs of dorsal neurons (DN1s and DN2s). CRY is present only in the PDF-expressing LNs and the DN1s. The larval visual organ expresses only two rhodopsins (RH5 and RH6) and projects onto the LNs. We recently showed that PDF signaling is required for light to synchronize the CRY−larval DN2s. We now show that, in the absence of functional CRY, synchronization of the DN1s also requires PDF, suggesting that these neurons have no direct connection with the visual system. In contrast, the fifth (PDF−) LN does not require the PDF-expressing cells to receive visual system inputs. All clock neurons are light-entrained by light–dark cycles in therh52;cryb,rh61cryb, andrh52;rh61double mutants, whereas the triple mutant is circadianly blind. Thus, any one of the three photosensitive molecules is sufficient, and there is no other light input for the larval clock. Finally, we show that constant activation of the visual system can suppress molecular oscillations in the four PDF-expressing LNs, whereas, in the adult, this effect of constant light requires CRY. A surprising diversity and specificity of light input combinations thus exists even for this simple clock network. |
| Databáze: | OpenAIRE |
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