Cloning and characterization of the human ADH4 gene
| Autor: | Jan-Olov Höög, Hans Jörnvall, Hedvig von Bahr-Lindström |
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| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Restriction Mapping Biology Homology (biology) Mice Exon Sequence Homology Nucleic Acid Gene cluster Genetics Consensus sequence Animals Humans Gene family Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Gene Base Sequence Alcohol Dehydrogenase Intron Promoter Exons General Medicine TATA Box Molecular biology Introns Rats Multigene Family |
| Zdroj: | Gene. 103:269-274 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(91)90285-j |
| Popis: | Human alcohol dehydrogenase (ADH) constitutes a set of isozymes and enzymes with different tissue and substrate specificities. The subunits are coded for by at least five gene loci, ADH1-ADH5. We now report the cloning and analysis of the human ADH4 gene coding for the class-II ADH with pi-subunits. The gene spans a region of 21 kb and is divided into nine exons and eight introns. The arrangement is the same as for all analyzed mammalian class-I genes, but the region covered is 50% larger than that in the human class-I genes. The nucleotide (nt) sequences of the exons, exon/intron boundaries and 5'- and 3'-untranslated regions were determined. The transcription start point (tsp) of the ADH4 gene was defined by primer extension and localized to a position 61 nt upstream from the ATG start codon. A TATA box and a CAAT element were identified by homology to consensus sequences for tsp. No DNA structures homologous to the glucocorticoid-responsive elements (GRE) present in the ADH2 gene were found in the upstream region of the ADH4 gene, but two structures with a 70% identity to the GRE consensus sequence were found at nonhomologous locations. The difference and the overall low degree of identity, 41%, of the upstream regions suggest different regulatory mechanisms for the class-I and class-II genes. |
| Databáze: | OpenAIRE |
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