Decreased expression of small-conductance Ca2+-activated K+ channels SK1 and SK2 in human chronic atrial fibrillation
| Autor: | Su-Juan Kuang, Hui-ming Guo, Chun-Yu Deng, Shaoyi Zheng, Qiu-Xiong Lin, Tao Yu, Ruo-Bin Wu, Xi-Yong Yu, Zhi-Xin Shan |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Patch-Clamp Techniques Small-Conductance Calcium-Activated Potassium Channels Cell Culture Techniques Action Potentials General Biochemistry Genetics and Molecular Biology SK channel Internal medicine Atrial Fibrillation medicine Myocyte Repolarization Humans Sinus rhythm Atrial Appendage Myocytes Cardiac Patch clamp General Pharmacology Toxicology and Pharmaceutics Ion transporter Ion channel Ion Transport Chemistry Atrial fibrillation General Medicine Middle Aged medicine.disease Immunohistochemistry Endocrinology Female |
| Zdroj: | Life sciences. 90(5-6) |
| ISSN: | 1879-0631 |
| Popis: | Aims Small-conductance Ca2 +-activated K+ (SK) channels are recognized as new ion channel candidates in atrial fibrillation (AF), with pivotal implications as novel drug targets due to their atrial-selective distribution in humans. The purpose of this study was to investigate whether SK channels and the Ca2 +-activated K+ current (IK,Ca) are involved in electrical remodeling of human chronic AF (cAF) and whether they display the differential distribution between the right (RA) and left atria (LA). Main methods The right (RAA) and left atrial appendage (LAA) myocytes were obtained from 29 sinus rhythm (SR) and 22 cAF patients. The IK,Ca and action potential (AP) were recorded using the patch-clamp technique. Three SK channel subtypes (SK1–3) expressions were assayed by western blot and real-time quantitative PCR analysis. Key findings The IK,Ca was decreased and its role in AP repolarization was attenuated in cAF, concomitant with a significant decrease in protein and mRNA levels of SK1 and SK2. In either SR or cAF, there was no difference in the IK,Ca density and protein and mRNA expression levels of SK1–3 between RAA and LAA myocytes. Significance Our results demonstrated that SK1 and SK2 are involved in electrical remodeling of cAF. SK1–3 and IK,Ca do not display the inter-atrial differential distribution in SR or cAF. These findings provide a new insight into mechanisms of electrical remodeling of human cAF. |
| Databáze: | OpenAIRE |
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