Delivery of molecular cargoes in normal and cancer cell lines using non-viral delivery systems
| Autor: | Sepehr Soleymani, Sepideh Shahbazi, Nooshin Haghighipour, Azam Bolhassani, Seyed Alireza Nadji |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Papillomavirus E7 Proteins Green Fluorescent Proteins Antineoplastic Agents Bioengineering Transfection Applied Microbiology and Biotechnology Flow cytometry Green fluorescent protein 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Humans Drug Carriers medicine.diagnostic_test Chemistry fungi HEK 293 cells General Medicine Flow Cytometry Lipids Molecular biology HEK293 Cells 030104 developmental biology Microscopy Fluorescence Cell culture Lipofectamine 030220 oncology & carcinogenesis Cancer cell Cell-penetrating peptide Biotechnology |
| Zdroj: | Biotechnology Letters. 40:923-931 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-018-2551-2 |
| Popis: | In this study, transfection efficiency of human papillomavirus (HPV) E7 DNA and protein constructs into HEK-293T normal cell line, and A549 and TC-1 tumor cell lines was evaluated by four delivery systems including supercharge GFP, hPP10 cell penetrating peptide, TurboFect and Lipofectamine using fluorescence microscopy and flow cytometry. The results indicated that Lipofectamine 2000 and TurboFect produced more effective transfection for GFP and E7-GFP DNA constructs in HEK-293T cells compared to in A549 and TC-1 cells (p |
| Databáze: | OpenAIRE |
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