The crystal structure of a Polerovirus exoribonuclease-resistant RNA shows how diverse sequences are integrated into a conserved fold
| Autor: | Quentin Vicens, Anna-Lena Steckelberg, Jeffrey S. Kieft, David A. Costantino, Jay C. Nix |
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| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
RNA Stability 1.1 Normal biological development and functioning Sequence Homology Computational biology Genome Viral Biology Polerovirus 03 medical and health sciences food Underpinning research Plant virus Exoribonuclease Report RNA pseudoknot Viral Nucleic acid structure RNA structure Molecular Biology 3' Untranslated Regions 030304 developmental biology Sequence (medicine) Subgenomic mRNA X-ray crystallography 0303 health sciences Genome Base Sequence 030302 biochemistry & molecular biology RNA Fold (geology) biology.organism_classification Dianthovirus viral RNA Luteoviridae Evolutionary biology Exoribonucleases Mutation exoribonuclease resistance RNA Viral Nucleic Acid Conformation Biochemistry and Cell Biology Crystallization Developmental Biology |
| Zdroj: | RNA (New York, N.Y.), vol 26, iss 12 RNA |
| Popis: | Exonuclease-resistant RNAs (xrRNAs) are discrete elements that block the progression of 5’ to 3’ exonucleases using specifically folded RNA structures. A recently discovered class of xrRNA is widespread in several genera of plant-infecting viruses, within both noncoding and protein-coding subgenomic RNAs. The structure of one such xrRNA from a dianthovirus revealed three-dimensional details of the resistant fold but did not answer all questions regarding the conservation and diversity of this xrRNA class. Here, we present the crystal structure of a representative polerovirus xrRNA that contains sequence elements that diverge from the previously solved structure. This new structure rationalizes previously unexplained sequence conservation patterns and shows interactions not present in the first structure. Together, the structures of these xrRNAs from dianthovirus and polerovirus genera support the idea that these plant virus xrRNAs fold through a defined pathway that includes a programmed intermediate conformation. This work deepens our knowledge of the structure-function relationship of xrRNAs and shows how evolution can craft similar RNA folds from divergent sequences. |
| Databáze: | OpenAIRE |
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