Adequacy of the tetrazolium test methodology for Stylosanthes capitata Vogel seeds
| Autor: | Amanda Carvalho Penido, Maria Alice Bento Ávila, Vitor Oliveira Rodrigues, Dennis Vinicius Lopes de Sousa, João Almir Oliveira, Ariadne Santos Oliveira |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
viability 04 agricultural and veterinary sciences lcsh:Plant culture Biology biology.organism_classification 01 natural sciences vigor Horticulture forage seeds sementes forrageiras Germination Stylosanthes Seedling viabilidade Capitata 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Dormancy lcsh:SB1-1110 Imbibition Agronomy and Crop Science Water content Seed testing 010606 plant biology & botany |
| Zdroj: | Journal of Seed Science v.40 n.4 2018 Journal of Seed Science Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABRATES Journal of Seed Science, Vol 40, Iss 4, Pp 435-441 |
| Popis: | The seed sector is increasingly dynamic and therefore demands rapid decision-making. The tetrazolium test is a rapid test that determines the viability of seeds, especially from species that show slow germination or dormancy. The methodology described in the Rules for Seed Testing for Stylosanthes sp. is not applied effectively to the Stylosanthes capitata Vogel species. Three seed lots of S. capitata seeds with different quality levels were used to fit the tetrazolium test methodology. In order to determine the physiological quality of seeds, the tests of germination, first count germination, emergence speed index, initial and final seedling emergence percentage, electrical conductivity, respiratory activity and seed moisture content were performed, besides the imbibition curve for the establishment of pre-imbibition periods of the seeds to perform the tetrazolium test. The pre-imbibition and staining periods of 6, 12 and 18 h, and 3, 6, 12 and 24 h at concentrations of 0.075%, 0.1% and 0.5% tetrazolium solution were tested. It was concluded that the 12-h pre-imbibition period and 6-h staining at the 0.1% tetrazolium solution is ideal for evaluating the viability of S. capitata seeds. |
| Databáze: | OpenAIRE |
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