Induction and suppression of cell lysis in an electrokinetic microfluidic system

Autor: Sanaz Habibi, Hwi Yong Lee, Hector Moncada‐Hernandez, Adrienne R. Minerick
Rok vydání: 2022
Předmět:
Zdroj: ELECTROPHORESIS. 43:1322-1336
ISSN: 1522-2683
0173-0835
DOI: 10.1002/elps.202100310
Popis: The ability to strategically induce or suppress cell lysis is critical for many cellular-level diagnostic and therapeutic applications conducted within electrokinetic microfluidic platforms. The chemical and structural integrity of sub-cellular components is important when inducing cell lysis. However, metal electrodes and electrolytes participate in undesirable electrochemical reactions that alter solution composition and potentially damage protein, RNA, and DNA integrity within device microenvironments. For many biomedical applications, cell viability must be maintained even when device-imposed cell-stressing stimuli (e.g., electrochemical reaction byproducts) are present. In this work, we explored a novel and tunable method to accurately induce or suppress device-imposed artifacts on human red blood cell (RBC) lysis in non-uniform AC electric fields. For precise tunability, a dielectric hafnium oxide (HfO
Databáze: OpenAIRE
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