First evidence of the mutations associated with pyrethroid resistance in head lice (Phthiraptera: Pediculidae) from Honduras

Autor: Carol Anahelka Rodríguez, Shabana Jamani, Kelsey Larkin, Ana Sanchez, Ariel Ceferino Toloza, Gonzalo Roca-Acevedo, Georgina Fronza
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Rural Population
Insecticides
Genome
Insect
Sodium Channels
law.invention
purl.org/becyt/ford/1 [https]
Human Head Louse
0302 clinical medicine
KDR
law
Genotype
Pyrethrins
Polymerase chain reaction
PEDICULUS HUMANUS CAPITIS
Genetics
Pediculus
Lice Infestations
Infectious Diseases
Pyrethroid
kdr
Restriction fragment length polymorphism
Polymorphism
Restriction Fragment Length
medicine.drug
Insecticide resistance
030231 tropical medicine
HONDURAS
Biology
PYRETHROID
lcsh:Infectious and parasitic diseases
03 medical and health sciences
parasitic diseases
medicine
Animals
Humans
lcsh:RC109-216
purl.org/becyt/ford/1.6 [https]
Alleles
Permethrin
VOLTAGE-SENSITIVE SODIUM CHANNEL
Pediculus humanus capitis
Point mutation
Research
Knockdown resistance
Genotype frequency
030104 developmental biology
Honduras
Amino Acid Substitution
Mutation
INSECTICIDE RESISTANCE
Parasitology
Voltage-sensitive sodium channel
Zdroj: Parasites & Vectors
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Parasites & Vectors, Vol 13, Iss 1, Pp 1-7 (2020)
ISSN: 1756-3305
Popis: Background: The human head louse, Pediculus humanus capitis, is a cosmopolitan blood-sucking ectoparasite afect‑ ing mostly schoolchildren in both developed and developing countries. In Honduras, chemical pediculicides are the frst line of treatment, with permethrin as their main active ingredient. Despite the extended use of these products, there is currently no research investigating insecticide resistance in Honduran head lice. In head lice, the most com‑ mon mechanism is knockdown resistance (kdr), which is the result of two point mutations and the associated amino acid substitutions, T917I and L920F, within the voltage-sensitive sodium channel (VSSC). Methods: Genomic DNA was extracted from 83 head lice collected in the localities of San Buenaventura and La Hicaca, Honduras. Polymerase chain reaction (PCR) was used to amplify a 332-bp fragment of the VSSC gene that contains a site afected by C/T mutation which results in a T917I amino acid substitution on each human head louse genomic DNA fragments. Results: The C/T non-synonymous mutation which results in the T917I kdr amino acid substitution was detected in both head lice populations at frequencies ranging between 0.45–0.5. Globally, the frequency of this substitution was 0.47. Of these, 5 (6.1%) were homozygous susceptible and 78 (93.9%) were heterozygotes. The kdr-resistant homozy‑ gote (RR) was not detected in the studied populations. Thus, 93.9% of the head lice collected in Honduras harbored only one T917I allele. Exact test for the Hardy-Weinberg equilibrium for both localities showed that genotype frequen‑ cies difered signifcantly from expectation. In addition, San Buenaventura and La Hicaca populations had an inbreed‑ ing coefcient (Fis) < 0, suggesting an excess of heterozygotes. Conclusions: To our knowledge, this is the frst study showing the presence of the C/T mutation responsible of the T917I kdr allele associated with pyrethroid resistance in P. h. capitis from Honduras. The PCR-restriction fragment length polymorphism (RFLP) employed here has demonstrated to be a reliable, economic, and reproducible assay that can be used to accurately genotype individual head lice for the mutation encoding the resistance-conferring T917I amino acid substitution. This highlights the necessity of proactive resistance management programmes designed to detect pyrethroid mutations before they become established within populations of head lice. Fil: Larkin, Kelsey. Brock University; Canadá Fil: Rodriguez, Carol A.. Universidad Nacional Autónoma de Honduras; Honduras Fil: Jamani, Shabana. Brock University; Canadá Fil: Fronza, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina Fil: Roca Acevedo, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina Fil: Sanchez, Ana. Brock University; Canadá. Universidad Nacional Autónoma de Honduras; Honduras Fil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Databáze: OpenAIRE
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