Translocation detection in lymphoma diagnosis by split-signal FISH: a standardised approach
| Autor: | David Y. Mason, Margaret Jones, Mette Christiansen, Jacques J.M. van Dongen, Stephen Hamilton Dutoit, Tim Svenstrup Poulsen, Maryse Baia, Christiane Copie Bergman, Steen Hauge Matthiesen, Evi Pouliou, José Cabeçadas, Dimitra Anagnostou, Juan F. García, Mateus Crespo, Anja Mottok, J. Han van Krieken, Anke van Rijk, Juan C. Cigudosa, Lorenzo Leoncini, Martin-Leo Hansmann, Mario Cocco |
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| Přispěvatelé: | Virology, Surgery, Immunology |
| Rok vydání: | 2008 |
| Předmět: |
Protocol (science)
Histology Lymphoma Chemistry(all) business.industry Lymphoma diagnosis Energy Engineering and Power Technology Hematology Computational biology Physics and Astronomy(all) Classification Bioinformatics Chromosomal aberration Pollution World health Pathology and Forensic Medicine Split-signal FISH Fuel Technology In situ hybridisation Validation Chemical Engineering(all) Medicine Fish Original Article business |
| Zdroj: | Journal of Hematopathology, 1, 119-126. Springer-Verlag Aarhus University Journal of Hematopathology van Rijk, A, Mason, D, Jones, M, Cabeçadas, J, Crespo, M, Cigudosa, JC, Garcia, JF, Leoncini, L, Cocco, M, Hansmann, ML, Mottok, A, Bergman, CC, Gaulard, P, Anagnostou, D, Pouliou, E, Hamilton-Dutoit, S J, Christiansen, MH, Poulsen, TS, Matthiesen, SH, van Dongen, J & van Krieken, JH 2008, ' Translocation detection in lymphoma diagnosis by split-signal FISH: a standardised approach. ', Journal of Hematopathology (Online), vol. 1, pp. 119-26 . |
| ISSN: | 1865-5785 |
| DOI: | 10.1007/s12308-008-0017-5 |
| Popis: | Lymphomas originating from the lymphatic system comprise about 30 entities classified according to the World Health Organization (WHO). The histopathological diagnosis is generally considered difficult and prone to mistakes. Since non-random chromosomal translocations are specifically involved in different lymphoma entities, their detection will be increasingly important. Hence, a split-signal fluorescence in situ hybridisation (FISH) procedure would be helpful in discriminating the most difficult classifications. The Euro-FISH programme, a concerted action of nine European laboratories, has validated a robust, standardised protocol to improve the diagnostic approach on lymphoma entities. Therefore, 16 fluorescent probes and 10 WHO entities, supplemented with reactive cases, were selected. The results of the Euro-FISH programme show that all probes were correctly cytogenetically located, that the standardised protocol is robust, resulting in reliable results in approximately 90% of cases, and that the procedure could be implemented in every laboratory, bringing the relatively easy interpretation of split-signal probes within the reach of many pathology laboratories. |
| Databáze: | OpenAIRE |
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