DNA-based detection of prostate cancer in urine after prostatic massage
| Autor: | Carsten Goessl, Mark Schrader, Rüdiger Heicappell, Markus Müller, Kurt Miller, Bernd Straub, Hans Krause |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Genetic Markers
Male PCA3 Pathology medicine.medical_specialty Urinalysis Urology Adenocarcinoma Urine Polymerase Chain Reaction Sensitivity and Specificity Prostate cancer Prostate medicine Humans Genes Tumor Suppressor Aged Glutathione Transferase Urine cytology Massage Intraepithelial neoplasia Palpation medicine.diagnostic_test business.industry Prostatic Neoplasms Cancer DNA Neoplasm DNA Methylation Middle Aged medicine.disease Isoenzymes medicine.anatomical_structure Glutathione S-Transferase pi Cancer research business |
| Zdroj: | Urology. 58:335-338 |
| ISSN: | 0090-4295 |
| DOI: | 10.1016/s0090-4295(01)01268-7 |
| Popis: | Objectives. Promoter hypermethylation of the glutathione-S-transferase P1 (GSTP1) gene is a specific feature of prostate cancer. This epigenetic DNA alteration served as the target for molecular detection of prostate cancer cells in urine sediments after prostatic massage. Methods. Bisulfite treatment followed by methylation-specific polymerase chain reaction was used to detect GSTP1 promoter hypermethylation in DNA isolated from urine sediments obtained after prostatic massage of men with and without prostate cancer. Results. GSTP1 promoter hypermethylation was demonstrated in the sediments of 1 (2%) of 45 patients diagnosed with benign prostatic hyperplasia, 2 (29%) of 7 patients with prostatic intraepithelial neoplasia, 15 (68%) of 22 patients with early, intracapsular cancer, and 14 (78%) of 18 patients with locally advanced or systemic prostate cancer, resulting in a specificity of 98% and an overall sensitivity of 73% for the detection of prostate cancer. Conclusions. Urinalysis for GSTP1 promoter hypermethylation constitutes a sensitive and highly specific DNA-based marker for molecular detection of prostate cancer, including early stages. |
| Databáze: | OpenAIRE |
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