In vivo immunological function of mast cells derived from embryonic stem cells: An approach for the rapid analysis of even embryonic lethal mutations in adult mice in vivo
| Autor: | Jochen Wedemeyer, Mindy Tsai, Leonard I. Zon, Stephen J. Galli, Soula Ganiatsas, See-Ying Tam |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Mutation
Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Cellular differentiation Stem Cells Biology Biological Sciences medicine.disease_cause Embryo Mammalian Embryonic stem cell Molecular biology In vitro Cell biology Transplantation Mice Inbred C57BL Mice Tissue engineering In vivo medicine Animals Mast Cells Stem cell Anaphylaxis Skin |
| Popis: | An important goal of tissue engineering is to achieve reconstitution of specific functionally active cell types by transplantation of differentiated cell populations derived from normal or genetically altered embryonic stem cellsin vitro. We find that mast cells derivedin vitrofrom wild-type or genetically manipulated embryonic stem cells can survive and orchestrate immunologically specific IgE-dependent reactions after transplantation into mast cell-deficientKitW/KitW-vmice. These findings define a unique approach for analyzing the effects of mutations of any genes that are expressed in mast cells, including embryonic lethal mutations,in vitroorin vivo. |
| Databáze: | OpenAIRE |
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