| Autor: |
Helena Lindgren, Helena Hedlund, Therese Graner, Pierre Le Greves, Lars Andersson, Staffan Lindqvist, Mattias Algotsson, Katarina Öberg, Lena Hörnsten, Marianne Carlsson, Maria Björner, Gunnar Glad |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Popis: |
Immobilized metal affinity chromatography (IMAC) purification of secreted histidine-tagged proteins in eukaryotic cell culture supernatants is often problematic. Incompatibility with the cell culture media appears as stripping of the immobilized metal ions required for binding of target proteins. The stripping effect is aggravated by low target protein concentrations and hence large sample volumes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
