Immunization with recombinant Helicobacter pylori urease decreases colonization levels following experimental infection of rhesus monkeys
| Autor: | Joseph E. Hill, James A. Boden, Jennifer Ingrassia, Kenneth F. Soike, Heather L. Gray, Weltzin Richard A, Cynthia K. Lee, Paul J. Giannasca, Thomas P. Monath, Thomas H. Ermak, Kathleen Georgakopoulos, Harold Kleanthous, James Blanchard, Timothy Tibbitts |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Spirillaceae
Bacterial Toxins Enzyme-Linked Immunosorbent Assay Helicobacter Infections Enterotoxins Adjuvants Immunologic Clarithromycin Metronidazole Organometallic Compounds medicine Animals Saliva Omeprazole Antibacterial agent Helicobacter pylori General Veterinary General Immunology and Microbiology biology Escherichia coli Proteins Public Health Environmental and Occupational Health biology.organism_classification Antibodies Bacterial Macaca mulatta Urease Recombinant Proteins Salicylates Anti-Bacterial Agents Vaccination Infectious Diseases Immunization Bacterial Vaccines Immunology biology.protein Molecular Medicine Drug Therapy Combination Gastritis medicine.symptom Antibody Bismuth medicine.drug |
| Zdroj: | Vaccine. 17:1493-1505 |
| ISSN: | 0264-410X |
| Popis: | Rhesus monkeys, naturally colonized with H. pylori as indicated by culture and histology were immunized with either 40 mg recombinant H. pylori urease administered orally together with 25 microg Escherichia coli heat-labile enterotoxin (LT) or immunized with LT alone. An initial 6 doses were administered over an 8 week period. All five vaccinated monkeys had a greater than two-fold rise in urease-specific serum IgG and IgA level and urease-specific salivary IgA was induced in 3 of 5 vaccinated animals after 6 or 7 doses of vaccine. Vaccination had no measurable therapeutic effect on H. pylori colonization. H. pylori was eradicated from these monkeys with a course of antimicrobials plus omeprazole, a 7th vaccine dose was given (10 months after the 6th dose) and they were rechallenged with H. pylori. Necropsy was performed 23 weeks after rechallenge and H. pylori colonization was determined by histological examination of 12 individual gastric sites. A significant reduction in colonization (p < or = 0.0001; Friedman's analysis of variance) was found in the vaccinated animals. Histopathologic examination of necropsy tissues also revealed a trend towards reduced gastritis and epithelial alterations in the vaccinated group compared to animals receiving LT alone. This study provides the first evidence for effective vaccination of nonhuman primates against H. pylori, and preliminary evidence that a reduction in bacterial density attributable to immunization may lessen gastric inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect