Frequency of donor cytotoxic T cell precursors does not correlate with occurrence of acute graft-versus-host disease in children transplanted using unrelated donors
| Autor: | Antonia Moretta, Eugenia Giraldi, Piero De Stefano, Marco Zecca, Daniela Montagna, Franco Locatelli, Rita Maccario, L. Prete, Patrizia Comoli, Cristina Daielli |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Adolescent Immunology Graft vs Host Disease Human leukocyte antigen Polymerase Chain Reaction Pathogenesis Antigen Immunopathology medicine Humans Immunology and Allergy Cytotoxic T cell Lymphocyte Count Typing Child Bone Marrow Transplantation HLA-D Antigens business.industry Histocompatibility Testing Incidence (epidemiology) Infant Tissue Donors medicine.anatomical_structure Child Preschool Acute Disease Female Bone marrow Lymphocyte Culture Test Mixed business T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Clinical Immunology. 16:107-114 |
| ISSN: | 1573-2592 0271-9142 |
| DOI: | 10.1007/bf01540957 |
| Popis: | Bone marrow transplantation (BMT) from unrelated volunteers is frequently associated with both increased incidence and increased severity of acute graft-versus-host disease (GVHD). In the last years, it has been suggested that the analysis of the frequency of cytotoxic T lymphocyte precursors (CTLp) of unrelated HLA-matched donors can be used to detect disparity for HLA class I antigens and as a predictive test for development of severe GVHD. In this report, we summarized our experience regarding 20 pediatric patients affected by various hematological disorders, receiving allogeneic BMT from unrelated donors. HLA class I and II antigens of donor/recipient pairs were determined by means of serological typing, whereas molecular typing of HLA-class II antigens of patients and their potential donors was performed using PCR-SSP and PCR-fingerprinting techniques. CTLp values, estimated using limiting dilution analysis, were high (range, 1∶7000–1∶40,000) in 9 of 20 patients, while the other 11 children had low or undetectable levels ( |
| Databáze: | OpenAIRE |
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