VGAT and VGLUT2 expression in MCH and orexin neurons in double transgenic reporter mice
| Autor: | Bingyu Zou, Priyattam J. Shiromani, Carlos Blanco-Centurion, Ying Sun, Emmaline Bendell, Meng Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genetically modified mouse SSC somatosensory cortex Transgene REM rapid eye movement Neuropeptide GAD67 glutamic acid decarboxylase-67 Article lcsh:RC321-571 03 medical and health sciences Glutamatergic MCH melanin concentrating hormone GABA 0302 clinical medicine NREM non-rapid eye movement Gad1 Glutamate decarboxylase 1 GAD65 glutamic acid decarboxylase-65 lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Reporter gene Chemistry General Neuroscience Glutamate receptor RTN reticular thalamic nucleus Orexin Cell biology CeA central nucleus of amygdala 030104 developmental biology nervous system VGAT vesicular GABA transporter VGLUT2 vesicular glutamate transporter-2 GABAergic GABA-γ Aminobutyric acid Glutamate Sleep Arousal hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery |
| Zdroj: | IBRO Reports IBRO Reports, Vol 4, Iss, Pp 44-49 (2018) |
| ISSN: | 2451-8301 |
| Popis: | Highlights • MCH neurons contain neither VGAT nor VGLUT2. • Majority of orexin neurons contain VGLUT2. • MCH neurons do not contain orexin. The neuropeptides orexin and melanin-concentrating hormone (MCH), as well as the neurotransmitters GABA (γ-Aminobutyric acid) and glutamate are chief modulators of the sleep-wake states in the posterior hypothalamus. To investigate co-expression of vesicular GABA transporter (VGAT, a marker of GABA neurons) and the vesicular glutamate transporter-2 (VGLUT2, a marker of glutamate neurons) in orexin and MCH neurons, we generated two transgenic mouse lines. One line selectively expressed the reporter gene EYFP in VGAT+ neurons, whereas the other line expressed reporter gene tdTomato in VGLUT2+ neurons. Co-localization between these genetic reporters and orexin or MCH immunofluorescent tags was determined using 3D computer reconstructions of Z stacks that were acquired using a multiphoton laser confocal microscope. Our results demonstrated that MCH neurons expressed neither VGAT nor VGLUT2, suggesting MCH neurons are a separate cluster of cells from VGAT+ GABAergic neurons and VGLUT2+ glutamatergic neurons. Moreover, most orexin neurons expressed VGLUT2, indicating these neurons are glutamatergic. Our data suggested that in the posterior hypothalamus there are four major distinct groups of neurons: VGAT+, orexin+/VGLUT2+, orexin-/VGLUT2+, and MCH neurons. This study facilitated our understanding of the role of these neurotransmitters and neuropeptides in relation to sleep/wake regulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|