Determination of alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats
| Autor: | Aytül Kürüm, Özkan Şimşek, Alev Gürol Bayraktaroğlu, Şevket Arikan, Emel Ergün |
|---|---|
| Přispěvatelé: | Kırıkkale Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
CATS General Veterinary biology Ziraat Mühendisliği lymphocyte Incubation period Kitten Staining Immune tolerance ANAE activity Angora cats stomatognathic diseases Endocrinology Immune system ANAE activity Angora cats lymphocyte pregnancy Veterinary Alpha-naphthyl Acetate Esterase Internal medicine biology.animal medicine Veteriner Hekimlik pregnancy Hormone |
| Zdroj: | Volume: 39, Issue: 1 57-61 Turkish Journal of Veterinary and Animal Sciences |
| ISSN: | 1300-0128 1303-6181 |
| Popis: | WOS: 000347678600009 This study aimed to determine the alpha-naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in pregnant, kitten, and adult Angora cats. In each group 9 healthy pregnant, adult, and kitten Angora cats constituted the material of the study. Optimum reaction was achieved after a 3 h incubation period at pH 5.8, by ANAE staining. Two types of reaction were observed in ANAE (+) T lymphocytes. The first reaction was a dot-like positivity pattern characterized by the presence of large granules, while the other was a granular positivity pattern characterized by the presence of 3 to 5 small granules. B lymphocytes reacted negatively in ANAE staining. The rate of ANAE (+) lymphocytes in pregnant, kitten, and adult Angora cats was 62.89 +/- 1.29%, 68.37 +/- 1.22%, and 77.71 +/- 1.63%, respectively. In conclusion, the lowest rate of ANAE-positive lymphocytes was detected in the pregnant cats, depending on the maternal immune tolerance supported by hormonal mechanisms. An increased rate of ANAE-positive lymphocytes was detected in the kittens, and the highest rate was measured in the adults, in parallel with the development of the immune system. |
| Databáze: | OpenAIRE |
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