A novel oncogene related to c-mil is transduced in chicken neuroretina cells induced to proliferate by infection with an avian lymphomatosis virus
| Autor: | C Béchade, Maria Marx, P Crisanti, Georges Calothy, Philippe Dezélée, D Laugier, Alain Eychène, B Pessac |
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| Rok vydání: | 1988 |
| Předmět: |
Molecular Sequence Data
Restriction Mapping Retroviridae Proteins Chick Embryo General Biochemistry Genetics and Molecular Biology Homology (biology) Virus Deoxyribonuclease EcoRI Oncogene Proteins v-raf Gene product Retrovirus Restriction map Transduction Genetic Animals Amino Acid Sequence Cloning Molecular Molecular Biology Gene Cells Cultured Electrophoresis Agar Gel Avian Leukosis Virus Base Sequence General Immunology and Microbiology Oncogene biology General Neuroscience DNA Oncogenes Cell Transformation Viral biology.organism_classification Virology Molecular biology Blotting Southern Research Article |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1988.tb03209.x |
| Popis: | Non-dividing neuroretina cells from chicken embryos are induced to proliferate after a long latency, following infection with Rous associated virus type 1, an avian retrovirus which does not carry a transforming gene. We have isolated from these proliferating cells an acutely mitogenic retrovirus, designated IC10, which contains a novel oncogene. Nucleotide sequencing showed that the IC10 virus has transduced 1101 nucleotides of cellular origin inserted between the gag and env genes of RAV-1. This oncogene, designated v-Rmil, is 70.1% homologous to v-mil. v-Rmil encodes a protein of 40,976 daltons sharing 83.8% homology with the catalytic domain of the v-mil protein. Divergence with the v-mil gene product is observed at the NH2- and COOH-terminal portions of the v-Rmil protein. Restriction analysis of normal chicken DNA indicated that v-Rmil is derived from a cellular gene distinct from c-mil. The c-Rmil gene is transcribed through a major mRNA, greater than 10 kb in length, that is detected at much higher levels in neuroretinas, as compared to other embryonic tissues. |
| Databáze: | OpenAIRE |
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