Mass Spectrometry Reveals High Levels of Hydrogen Peroxide in Pancreatic Cancer Cells

Autor: Qinlei Liu, Wenjie Ge, Sandra Martínez‐Jarquín, Yuliang He, Ri Wu, Markus Stoffel, Renato Zenobi
Rok vydání: 2023
Předmět:
Zdroj: Angewandte Chemie. International Edition, 62 (19)
ISSN: 1521-3773
1433-7851
0570-0833
Popis: Reactive oxygen species (ROS) are critical for many cellular functions, and dysregulation of ROS involves the development of multiple types of tumors, including pancreatic cancer. However, ROS have been grouped into a single biochemical entity for a long time, and the specific roles of certain types of ROS in tumor cells (e.g., pancreatic ductal adenocarcinoma (PDAC)) have not been systematically investigated. In this work, a highly sensitive and accurate mass spectrometry-based method was applied to study PDAC cells of humans and of genetically modified animals. The results show that the oncogenic KRAS mutation promotes the accumulation of hydrogen peroxide (H2O2) rather than superoxide or hydroxyl radicals in pancreatic cancer cells. We further identified that the enriched H2O2 modifies cellular metabolites and promotes the survival of pancreatic cancer cells. These findings highlight the specific roles of H2O2 in pancreatic cancer development, which may provide new directions for pancreatic cancer therapy.
Angewandte Chemie. International Edition, 62 (19)
ISSN:1433-7851
ISSN:1521-3773
ISSN:0570-0833
Databáze: OpenAIRE
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