Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content
| Autor: | Li Bugao, Zhao Yan, Chang Lu, Cai Chunbo, Yang Yang, Guo Xiaohong, Wei Hei, Gao Pengfei, Wanfeng Zhang, Cao Guoqing, Cheng Zhimin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adipogenesis
General Veterinary Physiology Chemistry Effector Adipose tissue Intramuscular Fat Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Lipid metabolism Transfection Animal Breeding and Genetics Lipid Metabolism Article Cell biology chemistry.chemical_compound QL1-991 Lipid droplet Adipocyte Genetics Animal Science and Zoology Zoology Gene Food Science Glutamic-oxaloacetic Transaminase 1 (GOT1) |
| Zdroj: | Animal Bioscience Animal Bioscience, Vol 35, Iss 2, Pp 155-165 (2022) |
| ISSN: | 2765-0235 2765-0189 |
| Popis: | Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation.Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content.Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p |
| Databáze: | OpenAIRE |
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