Enzymatic activity of mouse group X-sPLA2 improves in vitro production of preimplantation bovine embryos

Autor: Emilie Le Blévec, Jean-Pascal Hograindleur, Charles Coutton, Guillaume Martinez, Gérard Lambeau, Pascal Mermillod, Eric Schmitt, Pierre F. Ray, Christophe Arnoult, Louise Jeammet
Přispěvatelé: Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), IMV Technologies, Institut de pharmacologie moléculaire et cellulaire (IPMC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), CNRS, French National Research Agency (Agence Nationale de la Recherche) [ANR-14-LAB7-0004], Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), ARNOULT, CHRISTOPHE, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
In Vitro Oocyte Maturation Techniques
Embryonic Development
Reproductive technology
Phospholipase
sPLA(2)
Embryo development
Pla2g10
[SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction
Embryo Culture Techniques
03 medical and health sciences
Mice
0302 clinical medicine
Food Animals
Capacitation
medicine
Animals
Group X Phospholipases A2
Blastocyst
Small Animals
[SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology
030219 obstetrics & reproductive medicine
[SDV.BDLR.RS] Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction
Equine
Chemistry
0402 animal and dairy science
Embryo
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
04 agricultural and veterinary sciences
Bovine embryo
Oocyte
040201 dairy & animal science
Sperm
Spermatozoa
Cell biology
In vitro oocyte maturation
medicine.anatomical_structure
Fertilization
Oocytes
Animal Science and Zoology
Cattle
Female
Sperm Capacitation
Zdroj: Theriogenology
Theriogenology, Elsevier, 2019, 131, pp.113-122. ⟨10.1016/j.theriogenology.2019.03.030⟩
Theriogenology, 2019, 131, pp.113-122. ⟨10.1016/j.theriogenology.2019.03.030⟩
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2019.03.030⟩
Popis: International audience; Assisted reproductive technologies (ART) are widely used for both humans and domestic animals. In bovine species, in vitro embryo production is increasingly used and significant efforts are being made to optimize media and culture conditions. Phospholipase A2 (PLA2) are lipolytic enzymes that hydrolyze glycerophospholipids to produce free fatty acids and lysophospholipids that have been found to be critical for many biological processes. Mouse group X secreted PLA 2 (mGX) is abundant in the male reproductive tract and its use during sperm capacitation has been shown to improve in vitro production of viable embryos in a mouse model. Here, we examined its effect in the bovine species, testing the impact of mGX on the three steps involved in vitro production of preimplantation embryos: oocyte maturation, fertilization and preimplantation development. We found that incubating cumulus oocyte complexes (COC) or gametes with mGX resulted in increased blastocyst hatching and blastocyst production , respectively. The increases of embryo production induced by the phospholipase mGX were not observed for the catalytically inactive mutant H48Q-mGX, suggesting that these effects require the enzymatic activity of mGX. We also tested bGIB, a bovine homolog of mGX. bGIB failed to improve blastocyst production, underlining the high specificity of mGX. In conclusion, the results presented show that the effects of mGX are not restricted to the mouse model and that it is potent in the bovine species as well. This result strengthens the potential of mGX as a "pro-fertility drug" for mammalian reproduction.
Databáze: OpenAIRE
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