SARS-CoV-2 viral load dynamics and real-time RT-PCR cycle threshold interpretation in symptomatic non-hospitalised individuals in New Zealand: a multicentre cross sectional observational study
| Autor: | Gary McAuliffe, Sally A. Roberts, Fahimeh Rahnama, Kevin Smith, Andrew Fox-Lewis, Dragana Drinković, Susan Taylor, Blair Shilton, Kevin Howe, Catherine Jackson, Matthew Blakiston, Shivani Fox-Lewis, Susan C. Morpeth, Helen Qiao, Jenna Beaumont, Jay Harrower |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Adolescent Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Real-Time Polymerase Chain Reaction Asymptomatic cycle threshold Pathology and Forensic Medicine 03 medical and health sciences Young Adult 0302 clinical medicine COVID-19 Testing Virology Internal medicine medicine Humans Recent onset Child Aged Infectivity Aged 80 and over Cycle threshold business.industry Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 COVID-19 Middle Aged Viral Load PCR 030104 developmental biology Real-time polymerase chain reaction Cross-Sectional Studies 030220 oncology & carcinogenesis Child Preschool Observational study Female medicine.symptom business Viral load New Zealand |
| Zdroj: | Pathology |
| ISSN: | 1465-3931 |
| Popis: | We conducted a multicentre cross sectional observational study of laboratory, public health and hospitalisation data for PCR-confirmed COVID-19 cases within the New Zealand Northern Region, between 12 February and 8 June 2020. The aim of this study was to describe population level SARS-CoV-2 upper respiratory tract (URT) viral load dynamics by stratifying positivity rates and polymerase chain reaction (PCR) cycle threshold (Ct) values of URT samples from COVID-19 cases by days since symptom onset, and to explore utility of Ct values in determining length of time post-infection and thus potential infectivity. Of 123,124 samples tested for SARS-CoV-2 by PCR, 579 samples (407 positive and 172 negative) from 368 symptomatic non-hospitalised individuals with PCR-confirmed infection were included. Sample positivity rate was 61.5% (8/13) for pre-symptomatic samples, rising to 93.2% (317/340) for samples collected during the purported symptomatic infectious period (days 0–10 post-symptom onset), and dropping to 36.3% (82/226) for post-infectious period samples (day 11 onwards). URT viral load peaked shortly after symptom onset, with median Ct values ranging 20.00–29.99 until 15 days post-symptom onset, and >30.00 after this time. Of samples with a Ct value of |
| Databáze: | OpenAIRE |
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