A single DNA response element can confer inducibility by both alpha- and gamma-interferons
| Autor: | Dirk R. Gewert, Laurence E. Reid, Adrian H. Brasnett, Chris Gilbert, Ian M. Kerr, Andrew C. G. Porter, George R. Stark |
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| Rok vydání: | 1989 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Response element DNA Recombinant Biology Transfection Marker gene Interferon-gamma Exon Complementary DNA Gene expression Humans Gene family Amino Acid Sequence Gene Genetics Multidisciplinary Base Sequence DNA Cosmids Molecular biology Recombinant Proteins Regulatory sequence Multigene Family Interferon Type I Mutation Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 86:840-844 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.86.3.840 |
| Popis: | Genomic and cDNA clones corresponding to 9-27, a member of the human 1-8 gene family highly inducible by alpha- and gamma-interferons (IFNs), have been isolated and characterized. A 1.7-kilobase genomic clone contains a complete functional gene with two exons, encoding a 125-amino acid polypeptide of unknown function. The 5' flanking region of the gene contains a 13-base-pair IFN-stimulable response element (ISRE), homologous to the ISREs of the 6-16, ISG 15, and ISG 54 genes, which are predominantly inducible by IFN-alpha, beta. Analysis of constructs containing native and mutated ISREs suggests that this motif is essential for the response of 9-27 to IFN-gamma as well as IFN-alpha. Furthermore, the 9-27 (GGAAATAGAAACT) and 6-16 (GGGAAAATGAAACT) ISREs can each confer a response to both types of IFN when placed on the 5' side of a marker gene. Since the 6-16 gene does not normally respond to IFN-gamma, the context of the ISRE must determine the specificity of the response. |
| Databáze: | OpenAIRE |
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