Mechanisms of resistance to imipenem in imipenem-resistant, ampicillin-sensitive Enterococcus faecium
| Autor: | Christina Lundberg, Nagwa El Amin, Annelie Tjernlund, Bodil Lund, Bengt Wretlind, Kirsti Jalakas |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Imipenem Penicillin binding proteins Enterococcus faecium Drug resistance In Vitro Techniques Muramoylpentapeptide Carboxypeptidase Binding Competitive Pathology and Forensic Medicine Microbiology Antibiotic resistance Bacterial Proteins Ampicillin Drug Resistance Bacterial polycyclic compounds medicine Immunology and Allergy Humans Penicillin-Binding Proteins Binding Sites biology Strain (chemistry) General Medicine biochemical phenomena metabolism and nutrition Streptococcaceae biology.organism_classification Molecular Weight Hexosyltransferases Mutation Peptidyl Transferases Carrier Proteins Ampicillin Resistance medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0903-4641 |
| Popis: | Enterococcus faecium has six penicillin-binding proteins (PBP), where PBP5 seems to be the main target for beta-lactam antibiotics. The PBP profiles of three imipenem-resistant, ampicillin-sensitive E. faecium strains, isolated from the same patient, were studied using biotinylated ampicillin and chemiluminescence detection. Imipenem resistance in these strains was found to be associated with hyperproduction of PBP5 compared to the ampicillin- and imipenem-susceptible strain ATCC 19434. PBP5 in the imipenem-resistant strains (S1, B2) exhibited a selectively decreased affinity for imipenem. An 854 bp DNA fragment, corresponding to the penicillin-binding domain of pbp5fm, was studied in the resistant strains and the reference strain. Four amino acid substitutions were observed in the resistant strains compared to the susceptible one. The contribution of these substitutions to the increased production of PBP5 in these strains is unclear since the substitution was observed also in a strain without increased production of PBP5. Our results suggest that the moderate imipenem resistance observed in these strains is associated with increased production of PBP5 with relatively decreased affinity for imipenem, and that evolution of imipenem resistance in E. faecium is dinstinct from that of the other beta-lactams such as ampicillin. |
| Databáze: | OpenAIRE |
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