Developmental specificity of auxin response by pairs of ARF and Aux/IAA transcriptional regulators
| Autor: | Jill C. Wilmoth, Jason W. Reed, Alexandra Schlereth, Thorsten Hamann, Katja E Jäger, Gerd Jürgens, Marika Kientz, Dolf Weijers, Eva Benková |
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| Rok vydání: | 2005 |
| Předmět: |
Gravitropism
Arabidopsis Article General Biochemistry Genetics and Molecular Biology Auxin Gene expression Gene family heterocyclic compounds Promoter Regions Genetic Molecular Biology Transcription factor chemistry.chemical_classification Reporter gene Indoleacetic Acids General Immunology and Microbiology biology Arabidopsis Proteins General Neuroscience fungi Nuclear Proteins food and beverages biology.organism_classification Hypocotyl Cell biology chemistry Biochemistry Plant hormone Transcription Factors |
| Zdroj: | The EMBO Journal. 24:1874-1885 |
| ISSN: | 1460-2075 0261-4189 |
| DOI: | 10.1038/sj.emboj.7600659 |
| Popis: | The plant hormone auxin elicits many specific context-dependent developmental responses. Auxin promotes degradation of Aux/IAA proteins that prevent transcription factors of the auxin response factor (ARF) family from regulating auxin-responsive target genes. Aux/IAAs and ARFs are represented by large gene families in Arabidopsis. Here we show that stabilization of BDL/IAA12 or its sister protein IAA13 prevents MP/ARF5-dependent embryonic root formation whereas stabilized SHY2/IAA3 interferes with seedling growth. Although both bdl and shy2-2 proteins inhibited MP/ARF5-dependent reporter gene activation, shy2-2 was much less efficient than bdl to interfere with embryonic root initiation when expressed from the BDL promoter. Similarly, MP was much more efficient than ARF16 in this process. When expressed from the SHY2 promoter, both shy2-2 and bdl inhibited cell elongation and auxin-induced gene expression in the seedling hypocotyl. By contrast, gravitropism and auxin-induced gene expression in the root, which were promoted by functionally redundant NPH4/ARF7 and ARF19 proteins, were inhibited by shy2-2, but not by bdl protein. Our results suggest that auxin signals are converted into specific responses by matching pairs of coexpressed ARF and Aux/IAA proteins. |
| Databáze: | OpenAIRE |
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