Plasmid comparison and molecular analysis of Klebsiella pneumoniae harbouring blaKPC from New York City and Toronto

Autor: Nathan Kreiswirth, David A. Boyd, Kevin Katz, Stephen G. Jenkins, Donald E. Low, N. Prayitno, A. Gelosia, Michael R. Mulvey, Laura F. Mataseje, Susan M. Poutanen, Barbara M. Willey
Rok vydání: 2011
Předmět:
Male
Microbiology (medical)
Canada
Gene Transfer
Horizontal
Klebsiella pneumoniae
Microbial Sensitivity Tests
Biology
medicine.disease_cause
beta-Lactam Resistance
beta-Lactamases
Microbiology
Plasmid
polycyclic compounds
medicine
Pulsed-field gel electrophoresis
Humans
Pharmacology (medical)
Replicon
Escherichia coli
Aged
Aged
80 and over
Pharmacology
Molecular Epidemiology
Molecular epidemiology
Middle Aged
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
biology.organism_classification
Anti-Bacterial Agents
Bacterial Typing Techniques
Electrophoresis
Gel
Pulsed-Field
Klebsiella Infections
Molecular Typing
Multiple drug resistance
Infectious Diseases
Carbapenems
Multilocus sequence typing
Female
New York City
Transformation
Bacterial
Bacterial Outer Membrane Proteins
Multilocus Sequence Typing
Plasmids
Zdroj: Journal of Antimicrobial Chemotherapy. 66:1273-1277
ISSN: 1460-2091
0305-7453
Popis: OBJECTIVES This study examined Klebsiella pneumoniae clinical isolates and their bla(KPC) plasmids to determine potential relatedness of the isolates and their plasmids harbouring carbapenem resistance mechanisms. METHODS K. pneumoniae carbapenemase (KPC)-producing K. pneumoniae from New York City (NYC) (n = 19) and Toronto (n = 2) were typed by PFGE and multilocus sequence typing (MLST). bla(KPC)-harbouring plasmids were transformed into Escherichia coli DH10B(TM), restricted using EcoRI and analysed for bla content and replicon (rep) type. Susceptibility profiles for clinical and transformed strains were determined by automated microbroth dilution using CLSI breakpoints. Outer membrane protein (OMP) genes were analysed by sequencing of ompk35 and ompk36. RESULTS PFGE analysis identified 17 related strains (≥ 80% similarity; 11 KPC-2, 6 KPC-3) where ST258 was the dominant clonal type. All clinical isolates contained both bla(SHV) and bla(TEM-1) and, with the exception of one isolate, were multidrug resistant (MDR). Transformed KPC plasmids (n = 21) carried TEM-1 (n = 18) and were MDR (n = 5). Three plasmid clusters, repFIIA (n = 10), repR (n = 3) and an unknown type (n = 3), were observed. repFllA plasmids were observed from both NYC and Toronto strains. OMP gene analysis revealed premature stop codons in ompk35 and numerous deletions and insertions in ompk36. CONCLUSIONS The dissemination of bla(KPC) is due both to carriage of similar KPC-harbouring plasmids within genetically distinct K. pneumoniae and to clonal spread of K. pneumoniae with unrelated KPC plasmids.
Databáze: OpenAIRE
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