Role of tesmin expression in non‑small cell lung cancer
| Autor: | Aleksandra Piotrowska, Agnieszka Gomulkiewicz, Katarzyna Ratajczak, Jedrzej Grzegrzolka, Bozena Werynska, Mateusz Olbromski, Patrycja Sputa‑Grzegrzolka, Marzena Podhorska‑Okolow, Natalia Glatzel‑Plucinska, Adam Rzechonek, Piotr Dziegiel |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research proliferation Biology 03 medical and health sciences 0302 clinical medicine medicine Lung cancer non-small cell lung cancer Tissue microarray Oncogene minichromosome maintenance protein 5 Cancer Articles Cell cycle medicine.disease Molecular medicine minichromosome maintenance protein 7 lung cancer 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer cell tesmin Cancer research Immunohistochemistry |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | Non-small cell lung cancer (NSCLC) is the most commonly diagnosed cancer and the most frequent cause of cancer-associated mortality worldwide. Tesmin (MTL5) is a 60 kDa protein which has cysteine rich motifs, characteristic of metallothioneins. Tesmin expression was first observed in germ cells during spermatogenesis. Increased tesmin expression in NSCLC has been described previously. Minichromosome maintenance proteins (MCMs) serve a critical role in replication and cell cycle progression, i.e. in NSCLC. The aim of the present study was to evaluate the localization and intensity of tesmin, MCM5 and MCM7 protein expression in NSCLC and their association with the clinicopathological data of patients. Archival paraffin blocks of 243 cases of NSCLC and 104 non-cancerous tissue samples from the surgical margin (control) were obtained from patients treated at the Clinic of Thoracic Surgery of Wroclaw Medical University (Wroclaw, Poland) between 2010 and 2016, and were used for tissue microarrays and immunohistochemical (IHC) experiments. Laser capture microdissection was used for the isolation of cancer cells from 36 frozen samples of NSCLC and 8 control samples, and subsequently, MTL5, MCM5 and MCM7 mRNA expression was detected separately by reverse transcription-quantitative PCR. Positive cytoplasmic and nuclear tesmin, as well as nuclear MCM5 and MCM7 IHC expression were observed in 95.1, 83.67, 95.51 and 100% of the NSCLC cases, respectively. MTL5, MCM5 and MCM7 mRNA expression was observed in 91.66% of the cancer cases for all genes. The statistical analysis revealed increased tesmin IHC expression in cancer cells compared with the control. A positive correlation was observed between the IHC expression of nuclear tesmin and MCM5 proteins (r=0.33; P |
| Databáze: | OpenAIRE |
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