Structure and expression of wild-type and suppressible alleles of the Drosophila purple gene
| Autor: | John Yim, Christina Rosen, Dale Dorsett, Dongkook Park, Jaeseob Kim, Nacksung Kim |
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| Rok vydání: | 1996 |
| Předmět: |
Mutant
Molecular Sequence Data Gene Expression Biology Investigations Chromosome Walking Suppression Genetic Transcription (biology) Gene expression Genetics Animals Amino Acid Sequence Gene Alleles DNA Primers Messenger RNA Base Sequence Eye Color Sequence Homology Amino Acid Wild type Intron Molecular biology Alcohol Oxidoreductases RNA splicing Drosophila Phosphorus-Oxygen Lyases |
| Zdroj: | Genetics. 142(4) |
| ISSN: | 0016-6731 |
| Popis: | Viable mutant alleles of purple (pr), such as prbw, exhibit mutant eye colors. This reflects low 6-pyruvoyl tetrahydropterin (PTP) synthase activity required for pigment synthesis. PTP synthase is also required for synthesis of the enzyme cofactor biopterin; presumably this is why some pr alleles are lethal. The prbw eye color phenotype is suppressed by suppressor of sable [su(s)] mutations. The pr gene was cloned to explore the mechanism of this suppression. pr produces two PTP synthase mRNAs: one constitutively from a distal promoter and one in late pupae and young adult heads from a proximal promoter. The latter presumably supports eye pigment synthesis. The prbw allele has a 412 retrotransposon in an intron spliced from both mRNAs. However, the head-specific mRNA is reduced >10-fold in prbw and is restored by a su(s) mutation, while the constitutive transcript is barely affected. The Su(s) protein probably alters processing of RNA containing 412. Because the intron containing 412 is the first in the head-specific mRNA and the second in the constitutive mRNA, binding of splicing machinery to nascent transcripts before the 412 insertion is transcribed may preclude the effects of Su(s) protein. |
| Databáze: | OpenAIRE |
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