Dermal delivery of Fe-chlorophyllin via ultradeformable nanovesicles for photodynamic therapy in melanoma animal model
Autor: | Iman Gomaa, Mai Rady, Nagia N. Afifi, Mahmoud H. Abdel-Kader |
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Rok vydání: | 2018 |
Předmět: |
Skin Neoplasms
Light medicine.medical_treatment Melanoma Experimental Pharmaceutical Science Photodynamic therapy 02 engineering and technology Administration Cutaneous Metastasis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Animals Photosensitizer Cytotoxicity Skin Melanins chemistry.chemical_classification Drug Carriers Reactive oxygen species Photosensitizing Agents Chlorophyllides Chlorophyllin Melanoma Permeation 021001 nanoscience & nanotechnology medicine.disease Nanostructures Tumor Burden Mice Inbred C57BL Disease Models Animal Photochemotherapy chemistry 030220 oncology & carcinogenesis Cancer research 0210 nano-technology |
Zdroj: | International Journal of Pharmaceutics. 548:480-490 |
ISSN: | 0378-5173 |
DOI: | 10.1016/j.ijpharm.2018.06.057 |
Popis: | Melanoma is resistant to chemotherapeutics with poor prognosis and high potential of metastasis. Photodynamic therapy (PDT) represents a localized therapeutic modality, as cytotoxicity occurs when light activates photosensitizer (PS) at the tumour site. The aim of this study is dermal delivery of a high molecular weight hydrophilic photosensitizer (PS), ferrous chlorophyllin (Fe-CHL) via transethosomes for treatment of melanoma by PDT. Transethosomes were made of phosphatidyl choline, edge activator and 20% w/v Ethanol. They were evaluated for mean size, zeta potential, entrapment efficiency, ex-vivo permeation, localization in skin layers by transmission electron microscope (TEM), and finally, evaluated in melanoma animal model. Transethosomes of different mean vesicle size were evaluated for their skin retention and permeation through mice skin. TE of ∼500 nm (E3) being ultradeformable showed deep localization in skin confirmed by ex-vivo and TEM micrographs without permeation of PS to recipient compartment due to its size. The proposed study offers successful treatment of resistant melanoma by PDT, where complete tumour regression of small tumours occurred after single PDT, while large tumours after double PDT without recurrence for 8 months. This indicates the efficiency of nanovesicles in PS delivery and the efficiency of Fe-CHL in production of reactive oxygen species. |
Databáze: | OpenAIRE |
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